目的：研究微小RNA-34a（microRNA-34a，miR-34a）在人脑胶质瘤组织中的表达以及其对胶质瘤SHG-44细胞增殖和凋亡的影响。方法：20例胶质瘤组织标本均取自于青岛医学院附属医院神经外科（2007年01月至2010年12 月），作为对照的正常脑组织取自5位重症脑外伤需行减压手术的患者。Real-time PCR检测胶质瘤组织中miR-34a的表达。体外转染miR-34a mimics至SHG-44细胞中，MTT实验、流式细胞术检测SHG-44细胞的增殖、细胞周期及凋亡。结果：miR-34a在人脑胶质瘤组织中的表达量明显低于正常脑组织，其在Ⅲ、Ⅳ期胶质瘤组织中表达量明显低于Ⅰ、Ⅱ期胶质瘤组织。miR-34a mimics体外转染组与空白组相比，其细胞增殖抑制率明显提高\[（37.24±5.72）% vs（4.19±0.63）%，P<0.01\]；miR-34a mimics转染组SHG-44细胞G1期比例明显高于空白对照组\[（61.78±2.01）% vs（50.91±1.19）%，P<0.05\]；且miR-34a转染组细胞凋亡率与空白组细胞相比显著升高\[（15.28±3.65）%，vs（2.07±0.84）%，P<0.01\]。结论：miR-34a在人脑胶质瘤组织中低表达，miR-34a可抑制SHG-44细胞的增殖、诱导细胞周期阻滞和细胞凋亡。

Objective:To investigate the expression levl of microRNA-34a (miR-34a) in human glioma tissues, and further explore the role of miR-34a on proliferation and apoptosis of glioma SHG-44 cells. Methods: Twenty glioma samples were collected from the Department of Neurosurgery, Affiliated Hospital of Qingdao Medical College (January 2007 to December 2010). The normal brain tissues were obtained from 5 patients with severe traumatic brain injury who required post-trauma surgery. The expression level of MiR-34a in glioma tissues was detected by real-time PCR. After transfection of miR-34a mimics into SHG-44 cells, the proliferation, cell cycle and apoptosis were measured by MTT and flow cytometry, respectively. Results: The expression level of miR-34a was lower in the glioma tissues compared with the normal brain tissues, and miR-34a expression level was lower in the glima tissues of phase Ⅲ/Ⅳ than in phase Ⅰ/Ⅱ. The cell proliferation inhibitory rate increased signficantly in the miR-34a transfected group compared to the blank group (\[37.24±572\] % vs \[4.19±063\]%, P<0.01), the ratio of cells arrest at G1 phase was significantly higher in the miR-34a mimics transfected group compared to the blank group (\[61.78±2.01\]% vs \[50.91±1.19\]%, P<0.05), and the cell apoptosis in the miR-34a transfected group was significantly increased compared to the blank group (\[15.28±365\]% vs \[2.07±0.84\]%, P<001). Conclusion: MiR-34a was lowly expressed in human glioma tissues. MiR-34a can inhibit SHG-44 cell proliferation, thus inducing SHG-44 cell cycle arrest and promoting SHG-44 cell apoptosis.

山东省自然科学基金重点项目资助（No.Y2006C02）