目的： 探讨体外沉默Kruppel样因子4（Kruppel like factor 4， KLF4 ）基因的表达对食管癌KYSE140细胞增殖及迁移的影响。 方法: Western blotting法检测人食管癌细胞株KYSE140、KYSE150、EC109及EC9706及食管永生化细胞NE3中KLF4蛋白的表达，化学合成2对靶向 KLF4 的siRNA（KLF4-siRNA1，KLF4-siRNA2），并设对照siRNA（Ctrl-siRNA），分别体外转染至高表达 KLF4 的食管癌KYSE140细胞中，形成KLF4-siRNA1-KYSE140、KLF4-siRNA2-KYSE140 及Ctrl-siRNA-KYSE140细胞，通过MTT实验、Transwell实验分别检测转染后食管癌 KYSE140细胞的增殖及迁移。 结果: 食管癌细胞株KYSE140 中 KLF4蛋白的表达明显高于KYSE150、EC109及EC9706细胞株\[（5.62±0.02） vs （1.71±0.23）、（3.24±0.35）、（3.16±041），均P<005\]。KLF4-siRNA1-KYSE140、KLF4-siRNA2-KYSE140与Ctrl-siRNA-KYSE140细胞相比，KLF4蛋白表达明显降低\[（0.49±0.18）、（0.32±0.09） vs （0.98±0.19），均P<0.05\]，细胞增殖能力明显增高\[（1.2±0.8）、（1.4±0.1） vs （06±0.1），均P<005\]，迁移细胞数量也明显增加\[(780±22)、（475±25） vs （83±17）个，P<0.05\]。 结论： KLF4 在人食管癌细胞的增殖和迁移过程中起着负调控作用。

Objective:To investigate the effect of in vitro silencing Kruppel like factor 4 ( KLF4 ) gene expression on the proliferation and migration of esophageal cancer KYSE140 cells. Methods: Western blotting was used to detect the expression of KLF4 protein in the esophageal cancer cell lines, including KYSE140, KYSE150, EC109 and EC9706，and immortalized esophageal NE3 cells. Two pairs of siRNAs targeting KLF4 (KLF4-siRNA1，KLF4-siRNA2) and control siRNA (Ctrl-siRNA) were chemically synthesized, and were transfected into KYSE140 cells with a high expression of KLF4 in vitro to form KLF4-siRNA1-KYSE140, KLF4-siRNA2-KYSE140 and Ctrl-siRNA-KYSE140 cells. The proliferation and migration of esophageal cancer KYSE140 cells after transfection were detected by MTT assay and Transwell assay, respectively. Results: The expression of KLF4 in KYSE140 cells was higher than that in KYSE150, EC109 and EC9706 cells (\[562±0.02\] vs \[171±0.23\], \[3.24±0.35\], \[3.16±0.41\], both P<0.05). Compared with that in Ctrl-siRNA-KYSE140 cells, the expression of KLF4 protein was significantly decreased in KLF4-siRNA1-KYSE140 and KLF4-siRNA2-KYSE140 cells (\[0.49±0.18\], \[0.32±0.09\] vs \[0.98±0.19\], both P＜0.05), the capacities of proliferation were significantly increased (\[12±0.8\], \[1.4±0.1\] vs \[0.6±0.1\], both P<0.05), and the numbers of migration were also significantly increased (\[780±22\], \[475±25\] vs \[83±17\], P<0.05). Conclusion:KLF4 functions as a negative regulator in the proliferation and migration of esophageal cancer cells.

广东省自然科学基金资助项目（No. 10451008901005515, No. 10151008901000177）