目的： 观察N-Myc下游调节基因-2（N-Myc downstream-regulated gene 2, NDRG2）过表达对人膀胱癌T24细胞体外侵袭和迁移的影响。 方法： 采用携带NDRG2基因的慢病毒Lenti-NDRG2感染T24细胞，构建稳定过表达NDRG2的细胞株。Western blotting检测T24细胞中NDRG2、基质金属蛋白酶（matrix metalloproteinase，MMP）-2和MMP-9的表达，Transwell体外迁移和侵袭实验观察过表达NDRG2对T24细胞体外迁移和侵袭的影响。 结果： 建立了稳定过表达NDRG2的T24细胞株。与Lenti-Lacz组和空白对照组相比，Lenti-NDRG2组NDRG2蛋白的表达\[(30.1±1.27) vs (9.9±1.24)、(8.2±1.28)， P<001\]明显提高；Lenti-NDRG2组MMP-2 \[(13.5±1.32) vs (30.7±1.29)、(28.8±1.30), 均P<0.01\]和MMP-9 \[(11.7±127) vs (25.2±1.28)、(26.4±1.31), 均P<0.01\]的表达明显降低；Lenti-NDRG2组T24细胞的侵袭细胞数\[(18.1±3.4) vs (88.5±4.2)、(90.2±4.1)个，均P<0.01\]和迁移细胞数\[(20.1±3.5) vs (109.4±5.6)、(113.0±4.9)个，均P<0.01\]明显减少。 结论： 慢病毒介导的NDRG2基因过表达可能通过降低MMP-2和MMP-9的表达而抑制人膀胱癌T24细胞的转移与侵袭。

Objective: To observe the effect of NDRG2 (N-myc downstream-regulated gene 2) overexpression on invasion and metastasis of human bladder cancer T24 cell in vitro. Methods: The T24 cells were infected with lentivirus carrying NDRG2 gene (Lenti-NDRG2) to construct a stable cell line overexpression NDRG2.Western blotting was performed to examine the expression of NDRG2, matrix metalloproteinase (MMP)-2 and MMP-9 in T24 cells. The effects of NDRG2 overexpression on the invasion and migration of T24 cells were examined by the transwell invasion and migration assays, respectively. Results: The T24 cell line stable overexpression NDRG2was successfully constructed. Compared with the LEN-LacZ group and the control group, the NDRG2 expression was efficiently increased in the Lenti-NDRG2 group (\[301±1.27\] vs \[9.9±1.24\], \[8.2±1.28\]; P<0.01); the MMP-2 (\[13.5±1.32\] vs \[30.7±1.29\], \[28.8±1.30\]; P<0.01) and MMP-9 expressions (\[11.7±1.27\] vs \[25.2±1.28\], \[26.4±1.31\]; P<0.01) in T24 cells of Lenti-NDRG2 group were significantly suppressed; the capabilities of the invasion (\[18.1±3.4\] vs \[88.5±4.2\], \[90.2±4.1\]; P<0.01）and migration (\[20.1±3.5\] vs \[109.4±5.6\], \[113.0±4.9\]; P<0.01) of T24 cells were significantly suppressed in the Lenti-NDRG2 group. Conclusion: The lentivirus-mediated NDRG2 overexpression can suppress the invasion and migration of human bladder cancer T24 cells.

国家自然科学基金资助项目（No.31070681）