目的：探讨黑素瘤抗原（melanoma-associated antigen，MAGE）-A4及肿瘤抑制基因 P73 在乳腺癌组织和相应癌旁组织中的表达及其临床意义。方法：选取60例河北医科大学第四医院2007年9月至2007年12月乳腺癌住院患者，采用免疫组织化学法检测乳腺癌组织及相应癌旁组织中MAGE-A4和P73蛋白的表达，并分析其与乳腺癌临床病理特征的相关性。结果：乳腺癌组织中MAGE-A4、P73蛋白阳性表达率为63.3%（38/60）、43.3%（26/60），癌旁组织中MAGE-A4、P73蛋白阳性表达率为0（0/60）、85%（51/60）（均P<0.01）。乳腺癌组织中MAGE-A4蛋白的表达与P73蛋白的表达呈正相关（r=0316，P=0.014）。MAGE-A4蛋白的表达与乳腺癌患者的年龄、病理类型、组织学分级、临床分期、肿瘤大小、淋巴转移、雌激素受体（estrogen receptor，ER）表达、孕激素受体（progestrogen receptor，PR）表达均无相关性，但与C-erbB2蛋白的表达呈负相关（r=-0.259，P=0.046）。P73蛋白表达与乳腺癌患者的年龄、病理类型、组织学分级、临床分期、肿瘤大小、淋巴结转移、C-erbB2表达均无相关性，但与ER（r=0.274, P=0.034）和PR的表达呈正相关（r=0.262，P=0.043）。结论：乳腺癌组织高表达MAGE-A4和P73蛋白，且MAGE-A4与P73蛋白之间可能存在重要的相互调节机制。

Objective : To investigate the expressions of melanoma-associated antigen (MAGE)-A4 and tumor suppressor gene P73 in breast cancer tissues and paracancerous tissues and their clinical significance. Methods: Sixty patients with breast cancer from September 2007 to December 2007 in Fourth Hospital of Hebei Medical University were enrolled in the study. The expressions of MAGE-A4 and P73 proteins in breast cancer tissues and paracancerous tissues were detected by immunohistochemisty assay, and their correlations with the clinicopathological features of breast cancer were analyzed. Results: The positive expression rates of MAGE-A4 and P73 in breast cancer tissues were 63.3% (38/60) and 43.3% (26/60), respectively, while in adjacent tumor tissues were 0 (0/60) and 85% (51/60), respectively (P<001). In the breast cancer tissues, the expression of MAGE-A4 protein was positively correlated with P73 (r=0.316, P=0.014). MAGE-A4 protein expression was not correlated with patients’ age, pathological type, histological grade, clinical stage, tumor size, metastatic state of lymph node, estrogen receptor (ER), as well as progestrogen receptor (PR) status. However, MAGE-A4 protein expression was negatively correlated with expression of C-erbB2 protein (r=-0.259, P=0046). P73 protein expression was not correlated with patients’ age, pathological type, histological grade, clinical stage, tumor size, metastatic state of lymph node, and C-erbB2 expression. Whereas, the expression of P73 protein was positively correlated with the expression of ER (r=0.274, P=0.034) and PR (r=0.262, P=0.043). Conclusion: Breast cancer tissues highly express MAGE-A4 and P73 protein, and there exists an important regulatory mechanism between MAGE-A4 and P73 protein.

河北省自然科学基金资助项目（No. H2012206077）