目的：研究人乳腺癌候选抑癌蛋白1基因（breast cancer suppressor candidate 1，BCSC-1）过表达对肝癌Bel-7402细胞的增殖、侵袭、黏附和迁移的影响，并探讨其可能的机制。方法：将pcDNA3.1/v5-HisB-BCSC-1和空质粒pcDNA3.1/v5-HisB转染的Bel-7402细胞作为BCSC-1组和空载体组，Bel-7402细胞为野生型组。MTT法、Transwell实验、体外黏附实验和划痕实验分别检测BCSC-1过表达对Bel-7402细胞增殖、侵袭、黏附和迁移的影响，Real-time PCR检测BCSC-1过表达对Bel-7402细胞中与细胞增殖、黏附相关的 ICAM-1、PTTG、骨桥蛋白（osteopontin，OPN）mRNA表达的影响。结果：转染pcDNA3.1/v5-HisB-BCSC-1后Bel-7402细胞中 BCSC-1 mRNA的表达水平显著高于空载体组和野生组细胞\[（10.58±0.56）vs（1.10±022）、（1.00±0.01），均P＜0.01\]，成功制备 BCSC-1 稳定过表达的Bel-7402细胞株。与空载体组和野生型组相比， BCSC-1组Bel-7402细胞生长速度明显减慢\[72 h：（0.29±0.003） vs （0.34±0.014）、（0.35±0.013），均P<0.05\]；侵袭率\[（76.20±1.85）% vs（93.42±3.24）%、（100.00±1.05）%，均P<0.01）\]、黏附率\[（58.57±0.84）% vs（97.14±0.84）%、（100.00±130）%，均P<0.01\]显著降低，迁移距离显著降低\[（116.60±10.58） vs （231.33±10.26）、（237.96±11.58）μm，均P<001\]；同时过表达 BCSC-1的Bel-7402细胞的OPN mRNA表达量明显降低\[（0.12±0.06) vs (0.95±0.14）、（1.00±0.08），均P<001\]， ICAM-1、PTTG mRNA表达无明显变化。结论： BCSC-1过表达能够抑制Bel-7402细胞的增殖、侵袭、黏附和迁移能力，其机制可能与OPN基因表达下降有关。

Objective:To study the effects of overexpression of BCSC-1 gene on the proliferation, invasion, adherence and migration abilities of hepatoma carcinoma Bel-7402 cells, and to investigate the possible mechanism. Methods: Bel-7402 cells were transfected by pcDNA3.1/v5-HisB-BCSC-1 and pcDNA3.1/v5-HisB respectively as BCSC-1 group and empty vector group, and the wild-type Bel-7402 cells as a wild group. Proliferation, invasion, adherence and migration abilities of the Bel-7402 cells were detected by MTT assay, Transwell assay, adhesion experiment in vitro and scratch test, respectively. Real-time PCR was performed to detect the expression of BCSC-1 and osteopontin (OPN),  ICAM-1, PTTG mRNA, which were correlated with the cell growth and adhesion in Bel-7402 cells. Results: After transfected by pcDNA3.1/v5-HisB-BCSC-1, the expression of BCSC-1 mRNA in Bel-7402 cells was significantly raised compared with that of empty vector group and the wild-type group (\[10.58±0.56\] vs \[1.10±0.22\], \[1.00±0.01\]; all P＜0.01). The Bel-7402 cell line stably overexpressing BCSC-1 was successfully established. Compared with the empty vector group and wild group, the growth rate of Bel-7402 cells in the BCSC-1 group was obviously slower (72 h: \[0.29±0.003\] vs \[0.34±0.014\], \[0.35±0.013\]; all P<0.05), the invasion rate (\[76.20±1.85\]% vs \[93.42±3.24\]%, \[10000±105\]%; all P<0.01) and the adhesion rate (\[58.57±0.84\]% vs \[97.14±0.84\]%, \[100.00±130\]%; all P<0.01) were obviously decreased, the migratory distance was significantly reduced (\[116.60±10.58\] vs \[231.33±1026\], \[237.96±11.58\] μm; all P<0.01\]. The expression of OPN mRNA in the Bel-7402 cells which the BCSC-1 was up-regulated was significantly decreased (\[0.12±0.06\] vs \[0.95±0.14\], \[1.00±0.08\]; all P<001). No significant changes were observed in the expression of ICAM-1 and PTTG mRNA. Conclusion: Overexpression of BCSC-1 can inhibit the proliferation, invasion, adherence and migration abilities of Bel-7402 cells, which may be related to the decreased expression of OPN.

山东省自然科学基金资助项目（No. 2009CM019）；潍坊医学院大学生科技创新基金资助项目（No. KX2012009）