目的：研究新疆家蚕抗菌肽（cecropin-XJ,CE-XJ）与4种化疗药物联用对人食管癌Eca109细胞增殖及凋亡的影响。方法：MTT法检测CE-XJ联合不同浓度的多柔比星（doxorubicin，ADM）、卡铂（carboplatin,CBP）、顺铂（cisplatin，DDP）和环磷酰胺（cyclophosphamide，CTX）对人食管癌Eca109细胞增殖的影响，流式细胞术分析CE-XJ与ADM、CBP、DDP、CTX联用对Eca109细胞凋亡、活性氧及线粒体膜电位的影响。结果：单独使用CE-XJ（1～50 μmol/L）可依剂量依赖方式显著抑制Eca109细胞的增殖（ P <0.05或 P <0.01）。CE-XJ分别与ADM、DDP、CTX联合用药, 与单独用药相比，Eca109细胞的增殖抑制明显加强、细胞凋亡率显著增加（均 P <0.05或 P <0.01），细胞内活性氧水平显著增加（ P <0.05），而线粒体膜电位显著降低（均 P <0.05）。但CE-XJ与CBP联用时出现拮抗现象，与单独用药相比，细胞增殖抑制变化不大，细胞凋亡率反而降低。结论：CE-XJ与化疗药物ADM、DDP和CTX联用对人食管癌Eca109细胞的增殖抑制和凋亡诱导有明显促进作用，其机制可能与细胞内活性氧和线粒体膜电位变化有关。但CE-XJ与CBP联用则起拮抗作用，其机制有待进一步探讨。

Objective: To evaluate the effect of cecropin-XJ in itself or in combination with one of the commonly used chemotherapeutic agents on the proliferation and apoptosis of human esophageal carcinoma Eca109 cells in vitro. Methods：Eca109 cells were treated with cecropin-XJ, either in itself or in combination with adriamycin (ADM), cis-diamminedichloride platinum II (DDP) or cyclophosphamide (CTX). Twenty-four hours after treatment, cell viability was assessed by MTT assays, and apoptosis, reactive oxygen species (ROS) levels and mitochondrial membrane potential were assessed by flow cytometry. Results：Cecropin-XJ alone significantly inhibited Eca109 cell proliferation at the doses used (range from 1 to 50 μmol/L) (P<0.05). Compared with the cecropin-XJ mono-treatment, the combined treatment of cecropin-XJ with ADM, DDP or CTX were significantly more effective to induce Eca109 cell proliferation inhibition, apoptosis and reactive oxygen species production, and to decrease Eca109 cell mitochondrial membrane potential (P<0.05). In contrast, the combination of cecropin-XJ with CBP showed an antagonistic effect. Conclusion：Cecropin-XJ combined with ADM, DDP, and CTX respectively may inhibit proliferation and induce apoptosis of human esophageal carcinoma cells more effectively than cecropin-XJ in itself. The synergistic effect may be associated with changes in the intracellular ROS production and mitochondrial membrane potential.

新疆维吾尔自治区高技术研究发展计划项目（No.201110101）。