目的：检测巨噬细胞移动抑制因子（macrophage migration inhibitory factor, MIF）和VEGF在胃癌组织中的表达，分析其与胃癌临床病理特征之间的关系。方法：选取河南大学淮河医院2008—2013年手术切除的89例胃癌组织与2013年手术切除的新鲜胃癌及癌旁标本4对，分别采用免疫组织化学法和Western boltting方法检测胃癌组织及其癌旁组织中MIF和VEGF的表达，结合临床资料分析其表达水平与胃癌临床病理特征之间的关系。结果：MIF和VEGF在胃癌中的表达率分别为88.8%和50.6%，而在癌旁组织中均无表达。胃癌组织中MIF（0.87±0.29 vs 0.23±0.14，P<0.05）和VEGF（0.89±023 vs 0.34±0.21，P<0.05）相对表达量均显著高于癌旁组织。MIF和VEGF表达水平在不同年龄、性别组间无显著差异，而在不同分化程度和TNM临床分期组间差异有统计学意义（P<0.05）。结论：MIF和VEGF在胃癌组织中呈高表达，且可能与胃癌的分化程度和TNM临床分期有关。

Objective:To determine the expression of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor (VEGF) in gastric carcinoma in association with clinicopathological features of the carcinoma lesions. Methods: Eighty-nine tumor tissue specimens were collected between 2008 and 2013 and four pairs of tumor tissue and corresponding adjacent tissue specimens were collected in 2013 from patients who were diagnosed with gastric carcinoma in Henan University-Affiliated Hospital. Contents of MIF and VEGF proteins in gastric carcinoma and adjacent tissue specimens were assessed by immunohistochemical staining and Western blotting. The correlation of MIF and VEGF levels with clinicopathological features of gastric carcinoma was analyzed by statistical analysis. Results: MIF and VEGF were detected in 88.8% and 50.6% of gastric carcinoma respectively, but were undetectable in normal tissue specimens. In the four tissue pairs, gastric carcinoma tissue had significantly higher levels of MIF (0.87±0.29 vs 0.23±0.14, P<0.05) and VEGF (0.89±0.23 vs 0.34±0.21, P<0.05) compared with the corresponding adjacent tissue. The expression of MIF and VEGF was not associated with age and sex but was positively correlated with the histological grade and TNM stage of gastric carcinoma (P<0.05). Conclusion: MIF and VEGF are highly expressed in gastric cancer and the expression level is correlated with the cancer staging.