目的： 研究人食管鳞状细胞癌（esophageal squamous cell cancer, ESCC）组织及其癌旁组织中 c-Myc和 Bin1 基因的表达情况，并分析其临床意义。 方法： 收集2013年4月至2014年3月在河北医科大学第四医院胸外科行肿瘤切除并经病理证实的ESCC患者肿瘤组织和相应癌旁组织标本各54例，使用RT-PCR和免疫组织化学法检测ESCC组织和癌旁组织中c-Myc和 Bin1 基因的mRNA和蛋白的表达。 结果： 与癌旁组织相比，ESCC组织中的 c-Myc mRNA表达水平和蛋白阳性表达率均显著升高\[（0.34±0.29） vs （0.17±0.16）， P <0.001；55.56% vs 33.33%， P =0.033\]， Bin1 mRNA表达水平和蛋白阳性表达率均显著降低\[（0.25±0.19） vs （0.33±0.20）， P <0.001；57.41% vs 84.48%， P =0.007\]。ESCC组织中 c-Myc mRNA的表达与 Bin1 mRNA的表达呈显著负相关关系（ r =0.790， P <0.001），c-Myc蛋白表达与Bin1蛋白表达也呈显著负相关关系（ P =0.019）。c-Myc和 Bin1蛋白表达均与患者TNM分期、肿瘤侵犯深度、分化程度、淋巴结转移相关。 结论： 人ESCC患者肿瘤组织中c-Myc呈高表达，而Bin1呈低表达，两者表达呈负相关，均与ESCC进展和淋巴结转移有关联。

Objective : To investigate the expression of c-Myc and Bin1 in association with clinical characteristic in patients with esophageal squamous cell cancer (ESCC). Methods: Carcinoma and para-carcinoma tissue specimens were collected from 54 patients with esophageal squamous cell cancer who underwent esophagectomy in Fourth Hospital of Hebei Medical University between April, 2013 and March, 2014. Bin1 and c-Myc mRNA and protein levels were determined by RT-PCR and immunohistochemistry respectively. The associations between c-Myc and Bin1 protein levels and clinical features were analyzed. Results: Compared with para-carcinoma tissue specimens, carcinoma tissue specimens had significantly higher c-Myc mRNA abundance (0.34±0.29 vs 0.17±0.16, P <0.001) and a significantly higher percentage of c-Myc protein-positive tests (55.56% vs 33.33%, P =0.033). In contrast, Bin1 mRNA abundance was significantly decreased ( P <0.001) in carcinoma tissue specimens (0.25±0.19) as compared with para-carcinoma tissue specimens (0.33±0.20) and the percentage of Bin1 protein-positive tests was significantly lower in carcinoma specimens than in para-carcinoma specimens 57.41% vs (84.48%, P =0.007). The abundance of c-Myc mRNA was negatively correlated with Bin1 mRNA abundance ( r =-0.790, P <0.001) and c-Myc protein level was negatively correlated with Bin1 protein level ( P =0.019). Both c-Myc and Bin1 proteins were associated with TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis. Conclusion: In ESCC tissue, the expression of c-Myc is up-regualted while the expression of Bin1 is down-regulated. Bin1 is negatively correlated with c-Myc at both mRNA and protein levels. The aberrations in both c-Myc and Bin1 is closely associated with tumor invasion and lymph node metastasis of ESCC.

国家自然科学基金资助项目（No.81201607）；河北省杰出青年基金资助项目（No.H2014206320）；河北省自然科学基金资助项目（No.H2012206135）