目的：研究痰热清注射液对Lewis肺癌化疗模型小鼠免疫抑制作用的影响，以探讨其对荷肺癌小鼠的免疫调节作用。方法：构建C57 BL /6小鼠Lewis肺癌模型, 40只模型小鼠随机分为模型对照组、痰热清组、化疗组、痰热清联合化疗组及正常对照组，每组8只。治疗后摘小鼠眼球取血，取脾脏、外周血和胸腺，并分离腹腔巨噬细胞（Mφ），检测各组小鼠胸腺指数及外周血淋巴细胞数量，MTT法检测各组小鼠脾T、B淋巴细胞增殖水平，流式细胞术检测胸腺细胞的凋率和外周血内CD3+、CD3-NK1.1+细胞及CD3+NK1.1+细胞比例，RT-PCR检测胸腺组织 Bcl-2和Fas mRNA表达水平，乳酸脱氢酶（LDH）释放法和ELISA法检测Mφ的杀伤活性及及其分泌IL-12能力，免疫透射比浊法检测小鼠外周血清中IgG及补体C3的含量。结果：痰热清联合化疗组小鼠的胸腺指数、外周血淋巴细胞计数及T、B细胞增殖活性均明显高于单独化疗组（ P <0.01），同时胸腺细胞的凋亡水平明显低于单独化疗组（ P <0.01）。痰热清联合化疗组小鼠外周血CD3+T细胞、CD3-NK11+细胞及CD3+NK1.1+细胞比例均明显高于化疗组 ( P <0.01)。与化疗组相比，痰热清联合化疗组小鼠胸腺组织 Bcl-2 mRNA 水平明显增高，而 Fas mRNA水平明显减低(均 P <0.01)。痰热清组小鼠Mφ杀伤活性及分泌IL-12水平均明显高于化疗组(均 P <0.01) ，同时外周血IgG及补体C3含量明显高于化疗组(均 P <0.01)。结论：痰热清注射液对肺癌化疗小鼠的免疫系统有明显保护作用，其对抗肿瘤免疫力有明显的促进作用。

Objective:To investigate the effect of an injectable Chinese herbal medicine Tanreqing on chemotherapy- induced immunosuppression in a mouse model of lung carcinoma. Methods: A total of 40 C57 BL/6 mice were randomized to five groups: 1) normal control, 2) experimentally induced lung carcinoma, 3) Tanreqing treatment, 4) chemotherapy, and 5) chemotherapy combined with Tanreqing treatment. The designated treatments commenced 10 days after lung carcinoma induction and lasted for 5 days. At the end of the treatments, animals were sacrificed after periphery blood sampling, spleen and thymus were collected, and macrophages were isolated from peritoneal fluid. Thymus index and lymphocytes in peripheral blood were quantified. Apoptosis rate of thymus cells and proportion of CD3+, CD3-NK1.1+ and CD3+NK1.1+ cells in peripheral blood lymphocytes were determined by flow cytometry. The viability of T and B lymphocytes were assessed by MTT assay. Bcl-2 and Fas mRNA levels in thymus were measured by RT-PCR. The cytotoxicity of peritoneal macrophages and their secretion of IL-12 were determined with LDH and ELISA kits. Serum levels of IgG and C3 were measured by immune turbidimetry. Results: As compared with the chemotherapy group, the chemotherapy and Tanreqing combination group had significantly higher thymus index, lymphocyte count and T and B lymphocyte viabilities in the spleen ( P <0.01), significantly higher percentages of CD3+, CD3-NK1.1+ and CD3+NK1.1+ cells in peripheral-blood lymphocytes ( P <0.01), and significantly higher Bcl-2 mRNA abundance ( P <0.01) but significantly lower Fas mRNA abundance ( P <0.01).Peritoneal macrophages isolated from animals in the combination treatment group produced significantly higher levels of IL-2 and displayed significantly higher cytotoxicity than those isolated from animals in the chemotherapy group ( P <0.01). Moreover, serum levels of IgG and C3 were significantly higher in the combination group than in the chemotherapy group ( P <0.01). Conclusion: The injectable Chinese herbal medicine Tanreqing appears to have an immune protective and anti-tumor immunity-enhancing activities in lung carcinoma patients undergoing chemotherapy.

河北省中医药管理局基金资助项目（No.2013018）