目的：分析埃兹蛋白（ezrin）与CD44在肾细胞癌（renal cell carcinoma，RCC）中的表达情况及其临床意义。方法：选取2012年2月至2012年11月湖南省肿瘤医院泌尿外科收治的56例RCC患者的癌组织石蜡切片标本及17例癌旁组织标本，采用免疫组化技术检测标本中ezrin与CD44的表达情况，并分析两者表达的相关性及与RCC各临床病理指标的关系。结果：RCC中埃兹蛋白\[60.7%（34/56） vs 29.4%（5/17）， P <0.05\]与CD44蛋白\[64.3%（36/56） vs 0（0/17）， P <0.01\]表达水平明显高于癌旁组织。结合病理诊断结果研究发现，CD44蛋白和埃兹蛋白表达水平随RCC病理分级、临床分期的升高而升高（ P <0.05）；在肿瘤最大径>7.0 cm的RCC组织中，埃兹蛋白的阳性表达率显著高于最大径≤7.0 cm的RCC组织（ P ＜0.05 ），而CD44阳性表达率无显著差异（ P ＞0.05）。不同性别的患者组织内埃兹蛋白、CD44的表达水平无显著差异（ P >0.05 ）。Spearman分析结果表明，CD44与埃兹蛋白在RCC组织中的表达水平呈高度正相关（ r =0.427， P <0.01）。结论：埃兹蛋白与CD44在RCC组织中均呈高表达，两者表达呈正相关；埃兹蛋白与CD44在RCC的发生发展及浸润转移过程中扮演着重要角色。

Objective:The aim of this study was to analyze the expression at the protein level and clinic significance of ezrin and CD44 in renal cell carcinoma (RCC). Methods: Paraffin sections were prepared from renal cell carcinoma ( n =56 ) and adjacent tissue ( n =17) specimens collected from 56 RCC patients who were admitted to the Urology Department, Hunan Cancer Hospital between February 2012 and November 2012. Ezrin and CD44 proteins in these sections were assessed immunohistochemical staining. The correlation of ezrin and CD44 protein levels with the clinicopathological features of carcinoma lesions were analyzed. Results: Ezrin was detected in 60.7% (34/56) of RCC tissue specimens but in 29.4% (5/17) of the corresponding normal tissue specimens ( P <0.05). CD44 was detected in 64.3% (36/56) of RCC tissue specimens but in 0 out of 17 normal tissue specimens ( P <0.001). Both ezrin and CD44 proteins were correlated with the histological grade and clinical stage of RCC ( P ＜0.05). In addition, ezrin in tumors >7.0 cm was significantly higher than that in tumors ≤7.0 cm ( P ＜0.05) whereas CD44 was not significantly different between tumors >7.0 cm and tumors ≤7.0 cm (P＞0.05). The rates of both ezrin- and CD44-positive staining were not significantly different between specimens collected from male and female patients ( P >0.05). Ezrin was positively correlated with CD44 in RCC ( r =0.427， P <0.01). Conclusion: Both ezrin and CD44 are positively correlated with the severity of renal cell carcinoma, indicating these two proteins may play an important part in the development and progression of RCC.

湖南省卫生厅科研基金（No.B2013-095）