目的：观察胃癌术后患者DC联合细胞因子活化的杀伤细胞（cytokin-induced killer, CIK）治疗前后外周血中Treg细胞及相关细胞因子水平变化。方法：选择2009年4月至2013年12月在本院生物治疗中心治疗的胃癌术后患者158例，采用流式细胞术检测患者DC-CIK治疗前和治疗1～4个疗程后的胃癌术后患者外周血Treg（CD4+ CD25+ FoxP3+）细胞频率，ELISA检测血清Treg相关因子IL-10、TGF-β1水平。结果：接受3个疗程以上治疗（简称≥3疗程组）的75例患者的Treg细胞频率、IL-10、TGF-β1水平较治疗前均明显下降（均P<0.05），接受2个疗程以下治疗（简称≤2疗程组）的83例患者的Treg细胞频率、IL-10和TGF-β1水平较治疗前无明显变化。同一治疗组复发者的Treg细胞频率、IL-10、TGF-β1水平在复发时与未复发者比较无显著差异，但3个疗程以上治疗组的复发率明显低于2个疗程组（P<0.05）。结论：接受3个疗程以上DC-CIK治疗的胃癌患者其Treg频数、相应的细胞因子水平和复发率均明显下降，但同一治疗组复发与未复发者的Treg频数及细胞因子却无显著差异。

Objective: To assess changes in the proportion of CD4 + CD25 + FoxP3 + regulatory T (Treg) cells and their cytokine production in the peripheral blood of gastric cancer patients after immunotherapy with DC-CIK cells. Methods: This study enrolled 158 patients with gastric cancer who were admitted to the Biotherapy Center, Qingdao Center Hospital between April, 2009 and December, 2013. They received immunotherapy with DC-CIK cells for one to four cycles. At the end of the treatment, peripheral blood was collected. The proportion of Treg cells and levels of Treg cell-derived cytokines IL-10 and TGF-β1 were assessed by flow cytometry and ELISA respectively. Results: In patients (n=75) who received more than 3 cycles of immunotherapy, the proportion of Treg cells and levels of IL-10 and TGF-β1 were decreased significantly after DC-CIK therapy (Z＝-2.65, -3.21, -3.95, P<0.05). In patients who received less than 2 cycles of immunotherapy, the proportion of Treg cells and levels of IL-10 and TGF-β1 did not change significantly after treatment (P>0.05). In patients receiving the same number of cycle of treatment, the proportion of Treg cells and levels of IL-10 and TGF-β1 were not significantly different between recurrent and non-recurrence cases (P>0.05). Conclusion: Immunotherapy with DC-CIK cells for ≥3 cycles may significantly decrease the proportion of Treg cells and their cytokine levels in the peripheral blood. In the patients receiving treatment for the same number of cycles, recurrence has not effect on the proportion of Treg cells.

青岛市技术创新平台建设计划资助项目(No. 10-4-2-5-Jch)。