[关键词]
[摘要]
目的:研究人参皂苷Rg3是否是通过降低钙调蛋白(calmodulin, CaM)的表达来促进胃癌BGC-823细胞的凋亡及其可能的机制。方法:按加药的不同将BGC-823细胞,分为正常对照组、Rg3(50 μg/ml)组、Ad-CaM组和Rg3(50 μg/ml)+Ad-CaM组。MTT法检测Rg3和/或Ad-CaM对BGC-823细胞增殖的影响,流式细胞术检测Rg3和/或Ad-CaM对胃癌BGC-823细胞凋亡的影响,Transwell实验检测Rg3和/或Ad-CaM对胃癌BGC-823细胞侵袭能力的影响,Western blotting检测Rg3和/或Ad-CaM对胃癌BGC-823细胞内CaM、IκB 、CaMKⅡ和NF-κB表达的影响。结果: Rg3组可以明显促进胃癌BGC-823细胞的凋亡和抑制胃癌BGC-823细胞的增殖和侵袭,而Ad-CaM组和Rg3+Ad-CaM组明显抑制胃癌BGC-823细胞的凋亡和促进胃癌BGC-823细胞的生长和侵袭。Rg3组BGC-823细胞内NF-κB和CaMKⅡ的表达均明显抑制,而 IκB 的表达明显增强;Ad-CaM组和Rg3+Ad-CaM组BGC-823细胞内NF-κB和CaMKⅡ的表达均明显增强,而 IκB 的表达均明显抑制。结论: Rg3可能是通过降低CaM的表达来抑制NF-κB信号通路的活性,进而抑制胃癌BGC-823细胞的增殖和侵袭、促进其凋亡。
[Key word]
[Abstract]
Objective: To study whether and how ginsenoside Rg3 regulate apoptosis of gastric cancer BGC-823 cells. Methods: BGC-823 cells were treated with vehicle (control), Rg3 (50 μg/ml), Ad-CaM, and Rg3 (50 μg/ml) plus Ad-CaM, respectively. At 48 hours after treatment, cell viability was assessed by MTT assay, cell apoptosis by flow cytometry, cell invasive capacity by trans-well assay, CaM, Iκ B, CaMKⅡ and NF-κ B protein contents by Western blotting analysis. Results: Compared with vehicle control, Rg3 significantly promoted apoptosis and inhibited proliferation and invasion of BGC-823 cells (P<0.05), while Ad-CaM and Rg3+Ad-CaM significantly inhibited apoptosis and promoted proliferation and invasion of BGC-823 cells (P<0.05). At the protein level, the expression of NF-κ B and CaMKⅡ was significantly downregulated whereas the expression of IκBα was significantly in Rg3-treated BGC-823 cells (P<0.05), and the expression of NF-κ B and CaMKⅡ was significantly enhanced whereas the expression of IκBα was significantly inhibited in cells treated with Ad-CaM and Rg3 plus Ad-CaM respectively (P<0.05), as compared with control cells. Conclusion: Rg3 may inhibit proliferation and invasion and promote apoptosis of gastric cancer cells through downregulation of CaM kinase expression and inactivation of NF-κ B.
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[基金项目]
辽宁省科技厅自然科学基金资助项目(No. 2014022044);辽宁医学院校长基金资助项目 (No. XZJJ 20130230)。