目的：探讨RNA干扰技术靶向沉默膜联蛋白A7（anexin A7, ANXA7)的表达对人胃癌MGC-803细胞增殖和凋亡的影响。方法：实验分为3组，以靶向ANXA7的siRNA转染MGC-803细胞为siRNA干扰组，另设阴性siRNA对照和空白对照组。Western blotting检测转染后MGC-803细胞中ANXA7蛋白的表达，应用 MTT、集落形成和流式细胞术检测ANXA7下调对MGC-803细胞增殖、凋亡的影响。结果：靶向ANXA7的siRNA转染后MGC-803细胞中ANXA7的表达较阴性对照和空白对照组细胞明显降低［(21.79±1.72）% vs (99.87±5.13）%、(93.45±4.89）%，均 P <0.05］。下调ANXA7明显降低MGC-803细胞的增殖能力\[(0.97±0.06) vs (1.21±0.07)、(1.25±0.08)，均 P <0.05\]和集落形成能力\[(183±21) vs (363±35)、(348±27)个，均 P <0.05\]，细胞凋亡率无显著变化（ P >0.05）。结论：ANXA7低表达可抑制胃癌细胞MGC-803增殖，而对细胞凋亡无明显影响。

Objective: To investigate the effect of ANXA7 on the proliferation and apoptosis of human stomach carcinoma MGC-803 cells by downregulating ANXA7 expression using RNA interference technique. Methods: The MGC-803 cells were transfected with lipofectineTM2000 alone (blank control), scrambled siRNA (negative control), and siRNA targeted ANXA7 (experiment group). After 48 hours, ANXA7 expression was examined by immunoblotting. The proliferation and growth of transected cells were assessed by MTT and colony formation assays. Apoptosis of the cells was analyzed by flow cytometry. Results: The expression of ANXA7 was significantly decreased in cells transfected with ANXA7 siRNA compared with these in the two controls \[(21.79±1.72)% vs (99.87±5.13)% and (93.45±4.89)%, P <0.05\]. When ANXA7 expression was decreased, the proliferation and colony formation abilities of MGC-803 cells were markedly inhibited \[(0.97±0.06) vs (1.21±0.07) and (1.25±0.08), P <0.05; and (183±21) vs (363±35) and (348±27), P <0.05\], whereas the apoptosis of the cells was not affected statistically \[ P >0.05\]. Conclusion: ANXA7 regulates the proliferation and colony formation of MGC-803 cells, and appears not involved in their apoptosis.

河北省高校省级重点学科资助项目\[No.2013(4)号\]