目的：明确Neuritin在人非小细胞肺癌血管内皮细胞（non-small lung cancer-vascular endothelial cells, NSCLC-VECs）中的表达水平，探讨Neuritin与非小细胞肺癌血管发生的关系。方法：培养及鉴定人NSCLC-VECs及肺微血管内皮细胞株（human pulmonary microvascular endothelial cell, HPMEC），使用免疫荧光组织化学染色检测细胞膜上FactorⅧ抗体及CD34抗体表达水平，应用RT-PCR及Western blotting法检测Neuritin的表达水平。结果：经加压筛选稳定传代的NSCLC-VECs及HPMEC细胞膜上可见内皮细胞特异性标记物FactorⅧ抗体及CD34抗体表达。NSCLC-VECs中Neuritin mRNA的表达水平显著高于HPMEC\[（0.95±0.09） vs （0.64±0.05）， P<0.05\]，Neuritin蛋白在NSCLC-VECs中的表达的水平明显高于HPMEC\[（1.15±0.11） vs （0.27±0.14）， P <0.05\]。结论：Neuritin在人NSCLC-VECs中过表达，提示Neuritin可能与肿瘤血管发生相关，是一个潜在的肿瘤治疗靶点。

Objective: To investigatethe expression of Neuritin in non-small lung cancer-vascular endothelial cells (NSCLC-VECs) and its potential role in the development ofvessels in non-small lung cancer. Methods: We have cultivated and identified NSCLC-VECs and human pulmonary microvascular endothelial cells (HPMEC) by using anti-FactorⅧ and CD34 antibodies. The expression ofneuritin in these cells were examined through RT-PCR and immunoblotting. Results: After pressurized filtration, we obtainedstably passaged NSCLC-VECs and HPMECs, which express endothelial cells specific markers FactorⅧ and CD34. The level of neuritin mRNA in NSCLC-VECs is significantly higher than that of HPMEC\[(0.95±0.09） vs （0.64±0.05）， P <0.05\]. At protein level, neuritin expression in NSCLC-VECs is more than 4-fold higher than that in HPMEC\[(1.15±0.11） vs （0.27±0.14）， P <0.05\]. Conclusion： Neuritin isoverexpressed in human NSCLC-VECs andmay contribute to tumor angiogenesis. The differentialexpression also suggests that neuritinis a potential therapeutictarget fornon-small cell lung cancer.

新疆医科大学科研创新基金（No. XJC201159）