目的：分析食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）组织中含凝血酶敏感素1型基序的解聚素样金属蛋白酶（a disintegrin and metalloproteinase with thrombospondin motifs 1, ADAMTS1）及ADAMTS18基因的表达，探讨ADAMTS1和ADAMTS18基因在ESCC发生、发展中的作用。方法: 收集2008年6月至2011年8月河北医科大学第四医院手术切除的72例ESCC组织及癌旁组织，应用RT-PCR及免疫组化S-P法分别检测ESCC组织及癌旁组织中ADAMTS1、ADAMTS18 mRNA及其蛋白的表达。结果：①ESCC组织中ADAMTS1 mRNA的表达显著低于相应的癌旁组织\[（0.394±0.123） vs （0.895±0.276）， P <0.01\]，有淋巴结转移组ADAMTS1 mRNA表达显著高于无淋巴结转移组\[（0.482±0.157） vs （0.298±0.102）， P <0.05\]；②ESCC组织中ADAMTS18 mRNA表达显著低于相应的癌旁组织\[（0.361±0.115） vs （0.879±0.265）， P <0.01），高、中分化鳞癌组ADAMTS18 mRNA表达显著高于低分化鳞癌组\[（0.496±0.153) vs （0.232±0.088）， P <0.05\]；③ESCC组织中ADAMTS1和ADAMTS18蛋白阳性表达率显著低于癌旁组织\[38.9%（28/72）、34.7%（25/72） vs 94.4%（68/72）、93.1%（67/72），均 P <0.01\]，高、中分化组ADAMTS18蛋白阳性表达率显著高于低分化鳞癌组\[45.4%（20/44） vs 17.8%（5/28）， P <0.05\]；④ESCC组织中ADAMTS1与ADAMTS18的蛋白表达无明显相关性（ χ 2=1.338， P >0.05）。结论：ADAMTS1基因在ESCC中的异常表达可能与ESCC的发生及有无淋巴结转移有关；ADAMTS18基因在ESCC中的异常表达可能与ESCC的发生及组织分化程度有关；两种基因在ESCC组织中蛋白的表达不存在明显的相关性。

Objective: The present study was undertaken to examine the expression of ADAMTS1 and ADAMTS18 at mRNA and protein levels in esophagus squamous cell carcinoma (ESCC) for exploring their potential roles in the development and progress of the cancer. Methods: Tumor and adjacent noncancerous tissues were collected through surgical resection from 72 ESCC patients admitted to the Fourth Affiliated Hospital of Hebei Medical University between June 2008 and August 2011. The expression of ADAMTS1 and ADAMTS18 in ESCC tumor and adjacent tissues was examined with RT-PCR and immunohistochemistry to detect corresponding mRNA and protein. Results：①The level of ADAMTS1 mRNA in ESCC tumor tissues was significantly lower than that in adjacent non-tumor tissues (0.394±0.123 vs 0.895±0.276) ( P <0.01). Moreover, the level of ADAMTS1 mRNA in ESCC tumor tissues from patients with lymph node metastasis was significantly higher than that in ESCC tumor tissues from patients without lymph node metastasis (0.482±0.157 vs 0.298±0.102) ( P <0.05). ②The expression of ADAMTS18 mRNA in ESCC tumor tissues was significantly lower than that in adjacent non-tumor tissues (0.361±0.115 vs 0.879±0.265) ( P <0.01). Further, The mRNA level of ADAMTS18 mRNA in high/moderate differentiated ESCC was significantly higher than that in low differentiated ESCC (0.496±0.153 vs 0.232±0.088) ( P <0.05). ③The immunohistochemical positive rates of ADAMTS1 and ADAMTS18 in ESCC were significantly lower than that in adjacent non-tumor tissues \[38.9%(28/72) and 34.7%(25/72) vs 94.4%(68/72) and 93.1%(67/72) ( P <0.01)\]. Similar to its mRNA level, ADAMTS18 expression in high/moderate differentiated ESCC was significantly higher than that in low differentiated group \[45.4%(20/44) vs 17.8%(5/28) ( P <0.05)\]. ④There was no significant correlation between the expression of ADAMTS1 and ADAMTS18 in ESCC ( χ 2=1.338, P >0.05). Conclusion： Aberrant low expression of ADAMTS1 is associated with the development of ESCC and it lymph node metastasis, whereas reduced ADAMTS18 expression is closely related to ESCC development and its differentiation status. There are no correlation between the expression of ADAMTS1 and ADAMTS18 in ESCC.

河北省自然科学基金资助项目（No. H2015206196)。