目的：检测非小细胞肺癌（non-small cell lung cancer，NSCLC）患者外周血抗Hu抗体水平与健康志愿者抗Hu抗体水平进行比较，并分析其与肿瘤转移部位和多种临床病理变量间的关系。方法： 收集济南军区总医院肿瘤科2013年6月至2013年12月NSCLC患者外周血清标本75例，同时收集成年健康志愿者外周血清标本100例（除外肿瘤及神经系统疾病史）作为阴性对照，按照酶联免疫吸附试验原理，检测175例血清抗Hu抗体表达。纳入研究的变量主要包括：75例NSCLC患者的性别、年龄、组织类型、核分化级别、EGFR突变状态、肿瘤标志物（CEA、CY21-1和NSE）水平及远处转移部位（脑转移、骨转移及其他部位转移）。结果： 肺癌患者外周血抗Hu抗体水平\[（40.00±35.76) ng/ml\]显著高于健康人群\[（16.40±819) ng/ml, P=0.000\]。75例患者中有62.67%（47/75）抗Hu抗体高水平表达，37.33%(28/75)呈低表达。外周血抗Hu抗体水平与患者脑转移（P=0.015）显著相关；81.81%（9/11）的脑转移患者检测到高水平的抗Hu抗体，但仅仅50.00%（32/64）的非脑转移患者检测到高水平的抗Hu抗体；抗Hu抗体水平与患者性别、年龄、组织类型、分期、EGFR突变、CEA水平、CY21-1、NSE、骨转移及其他部位转移无关（P>0.05）。多变量回归分析显示，外周血中抗Hu抗体水平是预测脑转移的独立变量（P=0.015）。结论：非小细胞肺癌患者外周血中抗Hu抗体呈高水平，与脑转移显著相关。抗Hu抗体在非小细胞肺癌脑转移中的作用机制尚需进一步明确。

Objective : To investigate the clinical pathological features of patients with non-small cell lung cancer who had anti-Hu antibody and whether the presence of the antibody is associated with distant metastasis of the tumor. Methods: Peripheral blood samples were collected from 75 NSCLC patients enrolled into the Department of Oncology between June and December of 2013, and from 100 healthy volunteers who did not have tumor or neurologic history and underwent blood test between November and December of 2013 in the Clinical laboratory, General Hospital, Jinan Command of the People’s Liberation Army. Anti-Hu antibody was measured by ELISA. Clinical pathological features investigated include sex, age, pathological type, differentiation stage, EGFR mutation, tumor markers (CEA, CY21-1, and NSE), and distant metastasis. Results: Compared with healthy volunteers, NSCLC patients had significantly elevated anti-Hu antibody titration (40.00±35.76 ng/ml vs 16.40±8.19 ng/ml, P<0.01). The increase of the anti-Hu antibody was significantly associated with brain metastasis (81.81% vs 50.00%, P=0.015), but not distant metastasis to other tissues. It was also independent of sex, age, gender, pathological stage, EGFR mutation, and the levels of CEA and NSE (all P>005). Multivariate regression analysis further indicated that increased anti-Hu antibody level was an independent variable for brain metastasis prediction (P=0.015). Conclusion: Peripheral blood anti-Hu antibody is increased in NSCLC patients and is associated with brain metastasis of the tumor, which deserves further investigation to understand the mechanism.

国家自然科学基金项目资助项目（No.30901788; No.81272619)