目的：研究热激蛋白47（heat shock protein 47, HSP47）在人脑胶质瘤组织中的表达情况及其临床意义。方法：收集2008年1月至2009年12月沈阳军区总医院神经外科收治并手术的92例胶质瘤患者的肿瘤组织和同期手术切除病理诊断为胶质细胞增生的非胶质瘤组织10例，另有15例冻存的胶质瘤组织（收集于2014年5月至2014年6月），应用免疫组织化学方法、Western blotting检测其中HSP47蛋白的表达部位及表达水平，并结合随访资料分析HSP47蛋白表达与临床病理指标、患者生存期之间的关系。结果：HSP47阳性主要定位于Ⅲ、Ⅳ级胶质瘤细胞胞质和血管内皮细胞，HSP47在胶质瘤组织中表达率为52.17%、在非胶质瘤组织中无表达， χ2检验非肿瘤组和胶质瘤组之间HSP47表达的差异有统计学意义(χ2=9.855, P=0.002)。不同年龄、性别胶质瘤患者间HSP47蛋白的表达差异无统计学意义（P=0.423，P=0.820）； HSP47蛋白随着WHO病理级别增高阳性表达逐渐增多（P<0.05）。HSP47蛋白低表达组患者中位生存时间明显长于高表达组患者\[43（95% CI，22.4～63.6）vs 17（95% CI，14.5～19.5）个月，P<0.01\]。结论：HSP47在胶质瘤中过表达，并与病理级别呈正相关，高表达患者预后不良；HSP47可以作为胶质瘤诊断和治疗的新靶点。

Objective:To investigate the expression profile of heat shock protein-47 (HSP47) in glioma and evaluate the diagnostic and prognostic significance of HSP47 in clinical settings.Methods: Biopsy specimens were collected from 92 patients diagnosed with glioma and 10 patients diagnosed with gliosis between January, 2008 and December, 2009 at General Hospital of Shenyang Military Command. Additional 15 frozen glioma specimens collected between May, 2014 and June, 2014 were obtained. Immunoreactive HSP47 protein signals in these specimens were assessed by immunohistochemical staining and Western blotting. The prognostic significance of HSP47 was assessed by Kaplan-Meier survival analysis. Results:HSP47 was positive in 52.17% of glioma specimens but was undetectable in non-glioma specimens (χ2=9.855, P=0.002). In HSP47-positive Ⅲ、Ⅳ-grade glioma specimens, the immunoreative HSP47 protein signal was mainly located the cytoplasm of vascular endothelial cells. HSP47 expression was significantly associated with the pathological grade of the lesion (P<0.05) but not with gender (P=0.423) and age (P=0.820). The medium survival time was significantly longer in patients with low levels of HSP47 (43 months, 95 % CI, 22.4～63.6) than in patients with high levels of HSP47 (17 months, 95 % CI, 14.5～19.5) (P<0.001). Conclusion:HSP47 is overexpressed in glioma and its expression level is positively correlated with the pathological grade of the lesion. Our obervations suggest taht HSP47 may serve as a prognostic factor and a therapeutic target.

辽宁省自然科学基金资助项目（No.201202240)