目的：探讨上皮性卵巢癌（epithelial ovarian cancer，EOC）患者外周血、癌组织中KISS1 mRNA的表达及其临床意义。方法： RT-PCR 检测40例EOC、20例交界性卵巢肿瘤、20例良性卵巢肿瘤以及20例正常卵巢的组织及其术前静脉血中KISS1 mRNA的表达，分析KISS1 mRNA在4种组织间的表达差异性及在卵巢癌中的表达与临床病理特征的关系。结果：EOC组织及外周血KISS1 mRNA的表达量均高于良性肿瘤以及正常对照，差异有统计学意义（P<0.001）；EOC组织及外周血KISS1 mRNA的表达量与组织分化、临床分期、有无转移、术后残留病灶大小有关（P<0.05）；KISS1 mRNA在EOC原发病灶中的表达量高于其在对应转移灶中的表达，差异有统计学意义(P=0.001)；外周血与卵巢组织中KISS1 mRNA的表达水平呈正相关关系（r=0.669，P<0.001）。结论：KISS1基因可能参与了EOC的发生与发展，外周血KISS1 mRNA可作为卵巢癌分化程度、分期、复发及转移的生物学标志。

Objective : To investigate the expression of KISS1 in peripheral blood and carcinoma tissues of patients with epithelial ovarian cancer (EOC) and its clinical implication. Methods: RT-PCR was used to assess KISS1 mRNA level in ovarian tissues and peripheral blood of 40 patients with EOC, 20 patients with borderline epithelial ovarian tumors, 20 patients with epithelial benign tumors, and 20 patients with hysteromyoma. The differences of KISS1 expression among the 4 types of tissues and the relationship between KISS1 mRNA level and clinicopathologic features of EOC patients were further analyzed. Results: The level of KISS1 mRNA in carcinoma tissues and peripheral blood of patients with EOC was significantly higher than that in benign tumors and normal control group (P<0.001). The expression of KISS1 in cancer tissues and peripheral blood of patients with EOC was associated with the tumor grade, clinical stage, metastasis, and the size of post-operation residual tumor (P<0.05). The level of KISS1 mRNA in primary tumors of EOC was markedly elevated compared with that in the corresponding metastatic lesions (P=0.001). Furthermore, KISS1 expression in ovarian tissues was positively correlated to that in the peripheral blood（r=0.669，P<0.001）. Conclusion: KISS1 gene may be involved in the development and progress of EOC. Peripheral blood KISS1 mRNA may serve as a biomarker for the differentiation status , stage, recurrence, and metastasis of ovarian cancer.

南通市社会事业科技创新与示范计划项目资助（No.HS2013039）