目的：探讨金复康口服液对D-半乳糖诱导的免疫衰老模型小鼠肺癌移植瘤的防治作用。方法：采用SPSS 18.0软件包随机取10只C57BL/6J雄性小鼠作空白对照、取83只C57BL/6J雄性小鼠连续42 d颈背部皮下注射D-半乳糖\[150 mg/(kg·d）\]建立免疫衰老模型，分别以生理盐水和金复康口服液进行干预；造模后取10只对照组和 20只免疫衰老模型组小鼠，检测小鼠血清超氧化物歧化酶（SOD）活力和丙二醛（malondialdehyde, MDA)含量，摘取小鼠胸腺及脾脏，计算器官指数；流式细胞术检测小鼠胸腺及脾脏T细胞免疫衰老相关膜分子表达。其余63只免疫衰老模型小鼠左侧腋下前部接种肺癌细胞LL2-Luc-M38建立肺癌皮下移植瘤模型，并随机分为生理盐水、金复康预防和金复康防治组进行干预，其中30只（每组取10只）小鼠于移植瘤造模14 d后处死，检测瘤体质量；其余33只小鼠观察成瘤时间、计算生存期。结果：（1）与空白对照组相比，生理盐水组小鼠的脾脏指数、胸腺指数明显下降（P<0.05或P<0.01），小鼠胸腺和脾脏的CD3+CD45RA+、CD3+CD25+、CD3+CD28+表达显著下降（P<0.01），CD3+CD196+、CD4+CD25+表达显著上升（P<0.01），血清SOD活力明显下降（P<0.01），MDA含量明显上升（P<0.01）；金复康干预后，与生理盐水组相比，免疫衰老小鼠脾指数明显上升（P<0.01），脾CD3+CD45RA+和CD3+CD28+表达明显上升（P<0.01或P<0.05）、而CD3+CD196+及CD4+CD25+表达显著下降（P<001），胸腺CD3+CD25+表达显著上升（P<001）、CD3+CD196+表达显著下降（P<0.05），血清SOD活力明显上升（P<001），而MDA含量明显下降（P<0.01）。（2）免疫衰老小鼠皮下接种肺癌细胞后，金复康预防组小鼠的成瘤时间和生存期明显长于生理盐水组（P<0.05），金复康防治组的生存时间延长更为明显（P<0.01）；金复康防治组移植瘤体质量明显小于生理盐水组（P<0.01）和预防组（P<0.05），后两组间无明显差异（P＞005）。结论：D-半乳糖可诱导小鼠免疫衰老发生；金复康可时间依赖性地延缓小鼠免疫衰老，从而防治肺癌的发生发展及延长小鼠生存期。

Objective:To study the preventive and therapeutic effects of Jinfukang decoction on lung carcinoma in immunosenescence mouse induced by D-galactose. Methods:Using SPSS 18.0 software, 10 male C57BL/6J mice were randomly selected as control group and 83 male C57BL/6J mice as model group. In the model group, mice were subcutaneously injected with D-galactose (150 mg/kg/d) in scruff for 42 days to construct immunosenescence mouse model, normal saline and Jinfukang decoction were applied to intervene. After construction of the mouse model, 10 mice in the control group and 20 mice in the model group were selected to detect vitalities of serum superoxide dismutase (SOD) and contents of serum malondialdegyde (MDA),and then removed spleens and thymuses from the mice to calculate index of the organs; Flow cytometry was used to examine the expressions of immunosenscene related membrane molecules in T cells of the spleen and the thymuses. The rest 63 immunosenescence mice in the model group were subcutaneously injected with LL2-Luc-M38 lung cancer cells in the front of the left armpit to construct the model of Lewis lung cancer, and the mice were randomly divided into normal saline group, Jinfukang prevention group and Jinfukang prevention + treatment group. Among them, 30 mice (10 mice each group) were sacrificed at 14th day to measure the tumor mass, and the rest 33 mice were observed for time of tumor formation and survival time. Results: (1) Compared with the control group, the indexes of spleen and thymus in the normal saline group significantly decreased (P<0.05 or P<0.01); expression of CD3+CD45RA+, CD3+CD25+ and CD3+CD28+ in the spleen and the thymus significantly decreased (all P<0.01) while expression of CD3+CD196+ and CD4+CD25+ in the organs significantly increased (P<0.01); the vitalities of serum SOD remarkably decreased (P<0.001) while contents of serum MDA increased (P<0.001). After intervention of Jinfukang decoction, in the model group the spleen indexes increased significantly (P<0.01), expression of CD3+CD45RA+ and CD3+CD28+ increased (P<0.01 or P<0.05) and expression of CD3+CD196+ and CD4+CD25+ decreased (P<001) in the spleen, while expressioin of CD3+CD25+ increased (P<0.01), expression of CD3+CD196+ in the thymus decreased (P<0.05), vitalities of serum SOD increased (P<0.01) and contente of serum MDA decreased (P<0.01) compared with the normal saline group. (2) After the immunosenescence model mice were subcutaneously injected with lung cancer cells ,times of tumor formation and survival in the Jinfukang prevention group were significantly longer than those in the normal saline group (P<005), especially in the Jinfukang prevention + treatment group survival time was more significantly longer than that in the normal saline group (P<0.01); the tumor mass in the Jinfukang prevention + treatment group was smaller than those in the normal saline group (P<0.01) and the Jinfukang prevention group (P<005), but there was no significant difference between the latter two groups (P<0.05). Conclusion: The immunosenescence of mouse can be induced by D-galactose; Jifukang decoction can time-dependently delay the immunosenescence of mouse, by which Jifukang decoction can prevent the occurrence and development of lung cancer, and can prolong survival period of the mice bearing lung umor.

国家自然科学基金资助项目（No. 81173224，No. 81373621）；国家中医药管理局第一批全国中医药传承博士后资助项目（No. 135818）