目的：探讨树突状细胞(dendritic cell, DC)疫苗联合射频消融术（radiofrequency ablation, RFA）治疗结直肠癌肝转移（colorectal liver metastases, CRLM）患者的临床疗效。方法: 选取2012年8月至2014年8月在解放军第八一医院确诊并行RFA治疗的46例CRLM患者，其中26例进行DC疫苗联合RFA治疗（DC-RFA组），20例单纯进行RFA治疗（RFA组），比较两组患者近期、远期疗效，免疫功能，安全性及生活质量改善情况。结果: （1）DC-RFA组总有效率明显高于对照组（9231% vs 70.00%，P<0.05）；DC-RFA组与RFA组治疗后6个月生存率分别为96.15%、90.00%; 1、2年生存率DC-RFA组略优于RFA组（P>0.05）；（2）DC-RFA组外周血CD3+、CD4+、CD4+/CD8+百分比明显升高（P<0.05），CD8+数值降低（P>0.05）；RFA组治疗后外周血CD3+、CD4+及CD4+/CD8+百分比明显升高（P<0.05），CD8+数值升高（P>005）;（3）DC-RFA组治疗后仅有2例低热，1例过敏反应，对症处理后均恢复正常；（4）DC-RFA组患者生活质量有所提高，尤其是疼痛控制和精神状态方面。结论: DC疫苗联合RFA治疗CRLM患者可提高单纯RFA治疗的效果、延长生存期、提高机体免疫功能，同时可以有效改善患者生活质量，且治疗安全可靠。

Objective:To explore the clinical efficacy of dendritic cell vaccine combined with radiofrequency ablation (RFA) for treatment of colorectal liver metastasis (CRLM) patients. Methods: Forty-six patients with comfirmed CRLM underwent RFA in 81st Hospital of PLA from Aug. 2012 to Aug. 2014 were retrospectively analyzed, the patients were randomly divided into 2 groups: DC treatment combined with RFA group (n=26) and treatment group RFA control (n=20). After treatment, the two groups were compared for the recent curative effect, the long-term curative effect, immune function, safety and improvement in quality of life.Results：(1) The significant differences was found in the overall response rate between DC-RFA group and RFA group (92.31% vs 70.00%, P＜0.05); the six month survival rate of DC-RFA group and RFA group was 96.15% and 90.00% respectively. The 1- and 2- year survival rate of DC-RFA groups was slightly less better than that of RFA group（P＞0.05）; (2) in DC-RFA group, percentage of CD3+ and CD4+ the ratio of CD4+/CD8+ in peripheral blood increased significantly (P＜0.05), but CD8+ reduced (P＞0.05) after the treatment ; In the RFA group, percentage of CD3+and CD4+, the ratio of CD4+/CD8+ increased remarkably after treatment(P<005), while CD8+ slightly increased(P＞005). (3) In DC-RFA group, there were 2 cases of fever, 1 case of allergic reaction, and the patients recovered after treatment. (4) The life quality of the patients was improved in the DC-RFA group, especially in the aspects of mental state and pain control. Conclusion:In the treatment of patients with CRLM, DC vaccine combined with RFA can improve the therapeutic effect of RFA treatment, prolong the survival time, improve the immune function, and can effectively improve the quality of life and possess good treatment safety.