目的：探讨结缔组织生长因子 (connective tissue growth factor，CTGF)在卵巢癌组织中的表达及临床意义。方法： 采用免疫组化和逆转录聚合酶链反应(RT-PCR)方法检测105例卵巢癌、20例正常卵巢组织和30例良性卵巢肿瘤组织中CTGF的表达，并分析CTGF与卵巢癌临床病理参数的关系。结果：免疫组化检测观察到CTGF主要定位于细胞质内，卵巢癌、正常卵巢组织和良性卵巢肿瘤中CTGF的阳性表达率分别为53.33%（56/105）、95.00%（19/20）、93.33%（28/30），卵巢癌中CTGF阳性表达率低于正常卵巢（χ2=12.15,P=0.00)及良性卵巢肿瘤组织（χ2=15.88,P=0.00），正常卵巢与良性卵巢肿瘤组织相比较差异无统计学意义（χ2=0.05,P=0.80）,RT-PCR检测结果显示卵巢癌中CTGF mRNA的表达明显低于正常卵巢组织和良性卵巢肿瘤组织（F=3.39，P=0.039）。进一步采用χ2检验统计分析得出CTGF与卵巢癌的临床分期、淋巴转移相关（P<0.05），而与年龄、病理分级、病理类型及腹水癌细胞阳性率无相关性（P>0.05）。结论： CTGF在卵巢癌中低表达，与卵巢癌的发生、发展相关。

Objective:To investigate the expression and clinical significance of connective tissue growth factor (CTGF) in ovarian cancer. Methods: The expression levels of CTGF in 20 cases of normal ovarian tissues，30 cases of benign ovarian tumor tissues and 105 cases of ovarian cancer tissues were examined by RT-PCR and immunohistochemical staining, and the correlation between CTGF expression and clinicopathological significance of ovarian cancer was analyzed. Results: The positive rate of CTGF expression in normal ovarian tissues, benign ovarian tumor tissues and ovarian cancer tissues were 95.00%（19/20），93.33%(28/30) and 53.33%（56/105）respectively; the positive rate of CTGF in ovarian cancer tissues was significantly lower than that of normal ovarian tissues（χ2=12.15, P=0.00) and benign ovarian tumor tissues（χ2=15.88, P=0.00）; there was no significant difference in the positive rate between normal ovarian tissues and benign ovarian tumor tissues（χ2=15.88, P=0.00）. The CTGF mRNA expression in ovarian cancer tissue was lower than that in normal ovarian tissue and benign ovarian tumor tissue showed by RT-PCR ( F=3.39，P=0.039）. Data was processed by χ2 analysis, and the results showed that the expression of CTGF was correlated with clinical stages, lymph node metastasis（P<005）, but not correlated with age, pathologic type，pathologic grade and positive rate of ascites tumor cells (P>0.05）. Conclusion：CTGF was low expressed in overian cancer tissues and correlate with the occurrence and progression of ovarian cancer.

青岛市公共领域科技支撑计划资助项目（No.13-1-3-62-nsh）