目的：探索益气健脾抗癌法对大肠癌组织蛋白激酶C（protein kinase C；PKC）及亚型PKCδ、PKCε表达的影响及其作用机制。方法： 建立人大肠癌细胞HT-29裸鼠皮下移植瘤模型后，随机分为模型组、益气健脾组和益气健脾抗癌组。益气健脾中药由太子参、茯苓、白术、甘草、半夏、陈皮等组成，益气健脾抗癌中药由益气健脾中药加山慈菇、土茯苓、浙贝母和白花蛇舌草等组成。给药14 d后，应用Real-time PCR和Western blotting法检测肿瘤组织PKC及其亚型PKCδ、PKCε的mRNA及蛋白的表达。结果：益气健脾抗癌法治疗后，移植瘤组织中：（1）PKC mRNA和PKC蛋白表达低于模型组和益气健脾组\[（0412±0.040） vs （0.596±0.021）、（0.540±0.015）和（0.261±0.019） vs （0.665±0.016）、（0.498±0.018），P<0.05或P<0.01\]；（2）PKCδ mRNA和PKCδ蛋白表达高于模型组和益气健脾组\[（0.410±0.030） vs （0.233±0.025）、（0.261±0034）和（0.516±0.029） vs （0.301±0.041）、（0.361±0.044），均P<0.01\]；（3）PKCε mRNA和PKCε蛋白表达低于模型组和益气健脾组\[（0.215±0.021） vs （0.362±0.021）、（0.314±0.031）和（0.224±0.029） vs (0.368±0.044)、（0.359±0029），均P<0.01）。结论： 益气健脾抗癌法对大肠癌组织PKC、PKCε的抑制及PKCδ的促进作用，可能是益气健脾抗癌法治疗大肠癌的作用机制之一。

Objective:To study the influence of Yiqijianpi anticancer method on the expression of PKC (protein kinase C) and its subtypes (PKCδ and PKCε) in transplanted colon cancer tissues. Methods: After the establishment of subcutaneous xenograft model (human colon cancer cell line HT-29), the mice were randomly divided into 3 groups: model group, Yiqijianpi group, Yiqijianpi anticancer group. The Chinese herbs used in Yiqijianpi group were composed of Radix pseudostellariae, Poria, Atractylodes, Licorice root, Pinellia and dried orange peel etc. In addition to these, the herbs used in replenishing and anticancer group had additional Iphigenia, Tuckahoe, Zhejiang fritillaria, and Hedyotis diffusa willd. After 14 days of administration, the protein and mRNA expressions of PKC, PKCδ and PKCε were detected by RT-PCR and Western blotting. Results: （1） The mRNA and protein expressions of PKC were lower in the xenograft tissues of Yiqijianpi anticancer group than those of the model group and Yiqijianpi group (\[0.412±0.040\] vs \[0.596±0021\], \[0.540±0.015\] and \[0.261±0.019\] vs \[0.665±0.016\], \[0.498±0.018\]，P<0.05 , P<0.01)；（2）the mRNA and protein expressions of PKCδ were higher than those of the model group and Yiqijianpi group （\[0.410±0030\] vs \[0.233±0025\], \[0.261±0.034\] and \[0.516±0.029\] vs \[0.301±0.041\], \[0.361±0.044\]，all P<0.01);（3）the mRNA and protein expressions of PKCε were lower than those of the model group and Yiqijianpi group (\[0.215±0021\] vs \[0.362±0.021\], \[0.314±0.031\] and \[0.224±0.029\] vs \[0.368±0.044\], \[0.359±0029)，both P<0.01\].Conclusion: Yiqijianpi anticancer method had an inhibitory effect on PKC, PKCε and a promotive effect on PKCδ in transplanted colon cancer tissues, and this may be one of the mechanisms that this anticancer method works on colon cancer.

辽宁省中医药临床学（专）科能力建设资助项目(No.2013-Lnzyxzk-01)；辽宁省博士科研启动基金资助项目（No. 201501022）