目的：探讨食管鳞状细胞癌 (esophageal squamous cell cancer, ESCC) 患者的食管鳞癌组织、癌旁组织中 Bin1 基因启动子甲基化状态及其mRNA的表达及其临床意义。方法：采用实时荧光定量PCR(qRT-PCR)分别检测58例经病理证实的ESCC患者的食管鳞癌组织、癌旁组织中Bin1 基因mRNA的表达情况；用甲基化特异性PCR（MSP）检测上述食管鳞癌组织中 Bin1 基因启动子甲基化状态，比较ESCC患者Bin1甲基化状态与临床病理分期的关系。结果： ESCC组织中 Bin1基因启动子甲基化率明显高于癌旁组织(58.62% vs 25.86%, χ2=12.76, P<0.01)，Bin1甲基化状态与患者TNM分期、肿瘤侵润深度、分化程度、淋巴结转移相关（均P<0.05）。ESCC组织中 Bin1mRNA的表达水平明显低于癌旁组织\[（0.78±0.05） vs （1.03±0.03）, t=9.643, P<0.01）\]；发生Bin1甲基化的组织中Bin1 mRNA表达水平明显低于未发生甲基化的组织\[(0.68±0.04) vs (0.85±0.07）, t=2.476, P<0.05\]。结论：Bin1基因启动子区甲基化状态可能与ESCC的发生密切相关，它是ESCC中 Bin1mRNA低表达或缺失的机制之一，且与ESCC进展和淋巴结转移有关。

Objective:To detect the methylation status of Bin1 gene promoter and its mRNA expression in cancer tissues and para-carcinoma normal tissues from patients with esophageal squamous cell cancer (ESCC), and to explore its clinical significance. Methods: The expression level of Bin1 mRNA and its methylation status in ESCC tissues and the corresponding para-carcinoma normal tissues from 58 patients were determined by real-time quantitative RT-PCR (qRT-PCR) and methylation specific PCR (MSP). The relationship between Bin1 methylation status and clinical pathological staging was compared. Results: The methylation frequency of Bin1 in ESCC tissues was significantly higher than that in the corresponding para-carcinoma normal tissues (58.62% vs 25.86%, P<0.01), and the Bin1 methylation status was closely correlated with TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis (all P<0.05). Compared with para-carcinoma tissue specimens, the expression of Bin1mRNA in ESCC tissues was significantly lower (\[0.78±0.05\] vs \[1.03±0.03\], t=9.643, P<0.01) . The expression of Bin1 mRNA in ESCC tissues with methylation was significantly lower than that in ESCC tissues without methylation (\[0.68±0.04\] vs \[0.85±0.07\], t=2476, P＜0.05). Conclusion: Aberrant promoter methylation of Bin1 might be closely related to the development of ESCC, and it is one of the possible mechanisms that leads low expression or deletion of Bin1 in ESCC. The DNA methylation of Bin1 might be closely associated with tumor invasion and lymph node metastasis of ESCC.

国家自然科学基金资助项目（No.81201607）；河北省杰出青年基金资助项目（No.H2014206320）；河北省高层次人才资助项目（No.A201401040）