目的： 探讨下调microRNA-214（miR-214）表达对人卵巢癌SKOV-3细胞迁移和侵袭的作用及其可能机制。方法：采用脂质体介导法将miR-214抑制剂转染人卵巢癌SKOV-3细胞，Real-time PCR法检测miR-214、核因子-κB（nuclear factor-κB，NF-κB）和尿激酶型纤溶酶原激活剂（uridylyl phosphate adenosine，uPA）基因mRNA的表达，Western blotting法检测细胞NF-κB和uPA蛋白表达。划痕试验检测细胞迁移能力，Transwell小室法检测细胞侵袭能力。结果： 经转染miR-214抑制剂后，人卵巢癌SKOV-3细胞miR-214表达降低，细胞迁移和侵袭能力降低。同时NF-κB和uPA mRNA和蛋白表达也降低。结论：下调人卵巢癌SKOV-3细胞miR-214表达可抑制细胞迁移和侵袭能力,下凋NF-κB和uPA基因表达可能是其作用机制。

Objective:To observe effect of down-regulating microRNA-214 (miR-214) expression on migration and invasion of ovarian carcinoma SKOV-3 cells and explore its possible mechanism. Methods: miR-214 inhibitors were transfected into human ovarian carcinoma SKOV-3 cells by liposome. Expressions of miR-214, nuclear factor-κB (NF-κB) and urokinase-type plasminogen activator (uridylyl phosphate adenosine \[uPA\]) gene mRNA, as well as NF-κB and uPA proteins in the cells were respectively detected by Real-time PCR and Western blotting assays. Scarification test and transwell assay were used to detect abilities of migration and invasion of the cells respectively. Results: After transfection of miR-214 inhibitor, expression of miR-214 in human carcinoma SKOV-3 cells decreased, and migration and invasion abilities of the cells reduced. At the same time, expressions of NF-κB and uPA gene mRNA and proteins also depressed. Conclusion: Down-regulation of miR-214 expression in human ovarian carcinoma SKOV-3 cells could inhibit migration and invasion abilities of the cells, and down-regulation of NF-κB and uPA gene expressions might be its possible mechanism.

武警后勤学院创新团队课题资助(No.whtd2013-2）