目的：观察上皮间质转化（ epithelial-mesenchymal transition，EMT）相关因子 E-钙黏着蛋白（E-cadherin）和波形蛋白（vimentin）以及干细胞因子八聚体结合转录因子4（octamer-binding transcription factor 4，OCT4）在浸润性乳腺癌中的表达情况及OCT4与E-钙黏着蛋白和波形蛋白的相关性。方法：收集河北医科大学第四医院2009年手术切除的浸润性乳腺癌组织标本40例，实时荧光定量PCR和免疫组织化学法分别检测OCT4、E-钙黏着蛋白和波形蛋白的mRNA和蛋白的表达，分析其与临床病理特征的关系、OCT4和EMT相关蛋白之间的相关性以及与浸润性乳腺癌患者生存率之间的关系。结果：在浸润性乳腺癌组织中，OCT4、E-钙黏着蛋白和波形蛋白表达率分别为30%（12/40）、55%（22/40）和65%（26/40）。OCT4表达与浸润性乳腺癌患者的年龄、组织学分级、淋巴结转移以及Her-2的表达有关，E-钙黏着蛋白表达与淋巴结转移有关，波形蛋白表达与组织学分级和淋巴结转移有关。OCT4 mRNA和蛋白的表达与E-钙黏着蛋白和mRNA蛋白的表达呈负相关，与波形蛋白mRNA和蛋白的表达呈正相关，而E-钙黏着蛋白mRNA和蛋白的表达与波形蛋白mRNA和蛋白的表达也呈负相关。OCT4表达阳性患者的生存率明显低于表达阴性患者，差异具有统计学意义（P＜0.05）；而E-钙黏着蛋白和波形蛋白的表达与患者生存率之间未见明显相关性（P＞005）。结论：干细胞因子OCT4可能通过EMT促进浸润性乳腺癌细胞的转移，可能是影响浸润性乳腺癌预后的因素。

Objective:To detect expressions of mRNAs and proteins for EMT-assosiated factors, E-cadherin and vimentin, and stem cell factor OCT4 in invasive breast cancer and correlation of OCT4 with E-cadherin and vimentin. Method: Fourty samples of invasive breast cancer tissues were collected. Expressions of mRNAs and proteins for EMT-assosiated factors, E-cadherin and vimentin, and stem cell factor OCT4 were respectively detected by Real-time quantitative PCR and immune histochemistry assay. Relationship between expressions of the mRNA and the proteins, and survival rates and clinical pathological features of the patients with invasive breast cancer, and correlation of OCT4 with E-cadherin and vimentin, were analyzed. Results:In the invasive breast cancer tissues, expression rates of OCT4, E-cadherin and vimentin were 30% (12/40), 55% (22/40), and 65% (26/40) respectively. The expression of OCT4 was correlated with age, histological grade, lymph node metastasis and Her-2 expression of the patients with invasive breast cancer. Expression of E-cadherin was related with lymph node metastasis and expression of vimentin was related with histological grade and lymph node metastasis. Expressions of mRNA and protein for OCT4 and E-cadherin were negatively correlated. Expressions of mRNA and protein for OCT4 and vimentin were positively correlated. Also expressions of mRNA and protein for E-cadherin and vimentin were negatively correlated. Survival rates of the patients with positive expression of OCT4 were significantly lower than those of the patients with negative expression of OCT4 (P<0.05). However there were no significant differences between expressions of E-cadherin and vimentin, and survival rates of the patients (P>0.05). Conclusion: Stem cells factor OCT4 might promote metastasis of the invasive breast cancer cells via EMT, and might be a factor affecting prognosis of the invasive breast cancer.

国家自然科学基金青年基金资助项目（No. 81502032）