目的：探讨黑色素瘤相关抗原（melanoma antigen，MAGE）-As在食管鳞癌和贲门腺癌组织中的表达，分析其与食管鳞癌和贲门腺癌患者临床病理学特征及其预后的关系。方法：选取河北医科大学第四医院2010年9月至2010年11月住院患者手术切除的食管鳞癌和贲门腺癌组织及距离癌组织边缘5 cm以上且外观正常的相应癌旁组织标本各60例，同时选取5例该院前列腺癌住院患者术后的睾丸组织作为阳性对照，应用免疫组织化学法检测食管鳞癌和贲门腺癌组织及相应癌旁组织中MAGE-As蛋白的表达。结果：食管鳞癌和贲门腺癌组织中MAGE-As 蛋白表达率分别为 68.33%（41/60）和58.33%（35/60），而相应的癌旁组织未发现MAGE-As蛋白的表达。MAGE-As蛋白的表达与食管鳞癌患者的性别、年龄、临床分期、组织学分级、肿瘤大小、淋巴转移均无相关性（P>0.05）；MAGE-As蛋白表达与贲门腺癌患者的性别、年龄、临床分期、肿瘤大小、淋巴转移均无相关性（P>0.05），但与组织学分级呈正相关（P<0.05）。Log-Rank检验显示，MAGE-As蛋白表达阳性的食管鳞癌（P＝0.036）和贲门腺癌（P＝0.045）患者的生存期均显著低于表达阴性的患者。结论： MAGE-As蛋白是食管鳞癌和贲门腺癌的相关抗原，可作为食管鳞癌和贲门腺癌预后不良的指标。

Objective:To investigate the expressions of melanoma antigen (MAGE)-As in esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcioma (GCA), and to explore its correlation with clinical biological indicators and the prognosis in patients with ESCC and GCA. Methods: Cancerous (n=60) tissue samples and corresponding normal (n=60) adjacent tissues with normal morphology that 5 cm away from the lesions were collected from 60 patients with ESCC or GCA, who were surgically treated in Fourth Hospital of Hebei Medical University between September and November of 2010. In the meanwhile, testicular tissues (n=5) were collected from Men with prostate cancer as the positive control. Immunohistochemical staining was performed to assess the expressions of MAGE-As in carcinoma tissues and adjacent normal tissues. Results: The expression rates of MAGE-As protein in the tissues from ESCC and GCA were 6833% (41/60) and 58.33% (35/60) respectively, but it was not expressed in adjacent normal tissues. No correlation was found between MAGE-As protein expression and the age, gender, histological grade, clinical stage, tumor size, lymph node metastasis in patients with esophageal squamous cell carcinomas (P>0.05). MAGE-As protein expression was not correlated to the gender, age, clinical stage, tumor size, lymph node metastasis in patients with gastric cardia adenocarciomas (P>0.05), but positively related to the histological grade (P<0.05). Log-rank test showed that the survival time of ESCC patient (P=0.036) and GCA patients (P=0.045) with positive MAGE-As expression were significantly lower than those of the patients with negative expressions. Conclusion:MAGE-As proteins are the ESCC and GCA associated antigen. It may be regarded as a potential diagnostic and prognostic index in clinical settings.

河北省财政支撑项目资助(No.\[2012\]2056)；河北省科技支撑计划资助项目（No.14277732D）；河北省科技计划资助项目（No.152777184）