目的：检测人浆细胞瘤转化迁移基因1（plasmacytoma variant translocation 1, PVT1）在胃癌患者血液中的表达水平及临床常用肿瘤标志物的含量，探讨 PVT1 与肿瘤标志物和临床分期分级的关系。方法：采集2015年1月至12月石河子大学医学院一附院收住院的51例胃癌、63例慢性萎缩性胃炎患者与54名正常人外周静脉血，提取血液总RNA。实时定量PCR（qRT-PCR）技术检测血液中PVT1 mRNA 表达水平；电化学发光法检测胃癌患者血清中AFP、CEA、CA19-9的含量。结果：PVT1mRNA 在胃癌、慢性萎缩性胃炎患者血液中的表达量较高，胃癌组与正常组相比PVT1 mRNA表达量差异有统计学意义（t=0000，P<001），慢性萎缩性胃炎组与正常组相比PVT1 mRNA表达量差异也有统计学意义（t=0.000, P<0.01），但是胃癌患者与慢性萎缩性胃炎患者比较其差异无统计学意义（t=0.459，P>0.05）。PVT1 mRNA 表达与淋巴结转移有明显相关（r=0024, P<0.05）。 此外，PVT1mRNA的表达与血清肿瘤标记物CA19-9具有相关性（r=0.429，P<0.01）。结论：PVT1 mRNA在胃癌、慢性萎缩性胃炎患者血液中表达高于正常人，且与CA19-9具有相关性，提示 PVT1mRNA可能成为胃癌早期诊断及预后的分子标志物之一。

Objective:To examine expression level of human plasmacytoma variant translocation 1 (PVT1) gene and content of clinical commonly used tumor markers in blood of the patients with gastric cancer and to explore the relationship between PVT1 as well as tumor markers and clinical stages. Methods: Peripheral venous bloods of 51 cases with gastric cancer and 63 cases with chronic atrophic gastritis who hospitalized in the First Hospital Affiliated to School of Medicine, Shihezi University during January to December, 2015 as well as 54 normal persons were collected, from which total blood RNA was extracted. Real-time quatitative PCR (qRT-PCR) was used to detect expression levels of PVT1 mRNA in the bloods; contents of AFP, CEA and CA19-9 in the bloods of the patients with gastric cancer were examined by electrochemical luminescence assay. Results: Expression amounts of PVT1 mRNA in bloods of the patients with gastric cancer and chronic atrophic gastritis were higher, there was statistical difference of PVT1 mRNA expression amounts between gastric cancer group and normal group (t=0.000,P<0.01), there also was statistical difference of PVT1mRNA expression amounts between chronic atrophic gastritis group and normal group (t=0.000, P<0.01), but there was no statistic difference of PVT1 mRNA expression amounts between gastric cancer group and chronic atrophic gastritis group (t=0459, P>0.05). In addition, expression of PVT1 mRNAand serum tumor marker CA19-9 had correlation (r=0.429, P<0.01). Conclusion: Expression of PVT1 mRNA in blood of the patients with gastric cancer and chronic atrophic gastritis could be higher than that in blood of normal persons, and which might be correlated with CA19-9, indicating that PVT1mRNA might be as one of molecular markers for early diagnosis and prognosis.

新疆生产建设兵团应用基础研究资助项目（No.2015AG015）；石河子大学高层次人才研究项目（No. RCZX201332）