目的：探讨胰蛋白酶抑制剂sporamin对人胰腺癌细胞增殖、迁移和侵袭及Notch4蛋白表达的影响。方法:采用MTT法检测sporamin对人胰腺癌细胞株PANC-1和MiaPaCa-2增殖能力的影响，Transwell法检测胰腺癌细胞体外迁移和侵袭能力，Western blotting检测不同质量浓度的sporamin (0、25、50、75、100 μg/ml ) 对人胰腺癌细胞中Notch4蛋白表达水平的影响。结果: Sporamin能显著抑制人胰腺癌PANC-1和MiaPaCa-2细胞的增殖、迁移和侵袭能力，三种作用均呈显著质量浓度依赖性(P<005)，但无显著时间依赖性 (P>0.05)；随着sporamin质量浓度的增加，Notch4蛋白水平的表达也逐渐下降。结论: 胰蛋白酶抑制剂sporamin能质量浓度依赖性抑制人胰腺癌细胞增殖、迁移和侵袭能力，同时抑制人胰腺癌细胞中Notch4蛋白的表达。

Objective: To investigate effect of trypsin inhibitor, sporamin, on expression of Notch4 protein in human pancreatic cancer (PC) cells and proliferation, migration, invasion of the tumor cells. Methods: Effect of the sporamin on proliferation of the PANC-1 and MiaPaCa-2 PC line cells was detected by MTT assay; proliferation and invasion abilities in vitro of the pancreatic cancer cells detected by Transwell assay and effects of various concentrations of the sporamin (0，25，50，75 and 100 μg/ml) on expression level of the Notch4 protein in the human pancreatic cancer cells detected by Western blotting assay. Results: Sporamin significantly inhibited prolilferation, migration and invasion abilities of the PANC-1and MiaPaCa-2 PC line cells, as a patern of concentration-dependence (P<0.05), but not a patern of time-dependence (P>0.05). With increase of the sporamin concentration, expression level of the Notch4 protein also decreased gradually. Conclusion:Trypsin inhibitor sporamin could inhibit proliferation, migration and invasion abilities of the PC cells with a concentration-dependence, and simualtaneously inhibit expression of the Notch4 protein in the PC cells.

浙江省自然科学基金资助项目（No.LY16H160033）；浙江省公益技术应用研究项目（No.2016C33189）