目的：探讨自体肿瘤抗原负载树突状细胞联合细胞因子诱导的杀伤细胞(DC-CIK)治疗胃癌的疗效及安全性。方法： 回顾性分析2005年1月至2010年1月在福建省肿瘤医院行胃癌切除术患者270例，根据是否进行DC-CIK治疗，分为对照组（150例）和DC-CIK治疗组（120例）。应用流式细胞术分析DC-CIK细胞表型，比较两组患者的总生存期（OS），观察DC-CIK治疗的不良反应，Cox回归法分析胃癌预后影响因素。结果：成熟DC中HLA-DR+、CD8+、CD83+和CD86+的细胞比例分别为（96.16±1.51）%、（96.58±2.66）%、（96.44±2.20）%和（98.74±0.76）%。CIK中CD3+、CD3+CD4+、CD3+CD8+和CD3+CD16+/CD56+的细胞比例分别为（91.98±5.9）%、（25.19±10.5）%、（71.15±7.8）%和（18.73±74）%。DC-CIK治疗组中位OS为 77个月，与对照组的51个月相比差异明显（χ2＝4.431，P=0.035）。DC-CIK治疗、辅助化疗和TNM分期是胃癌预后的独立影响因素。DC-CIK治疗的常见不良反应为发热、寒战和乏力。结论：胃癌术后应用自体肿瘤抗原负载DC-CIK治疗可延长胃癌患者的OS，且不良反应轻，安全有效。

Objective:To explore the curative effect and safety of autologous tumor antigen loaded dendritic cells combined with cytokine-induced killer (DC-CIK) cells in the treatment of patients with gastric cancer. Methods: Two hundred seventy patients who received operative resection of gastric cancer in Fujian Provincial Cancer Hospital during January, 2005 to January, 2010 were retrospectively analyzed. Acording to wether receiving DC-CIK therapy, the patients were divided into DC-CIK treatment group (120 cases) and control group (150 cases). Flow cytometry assay was used to analyze phenotype of DC-CIK cells. Overall survival (OS) between both groups was compared. Advers reactions of the DC-CIK therapy were observed. And prognostic factors of the gastric cancer were analyzed by Cox regression model. Results: In the mature DC, the percentages of HLA-DR+, CD8+, CD83+ and CD86+ cells were（96.16±151）%, （96.58±2.66）%, （96.44±2.20）%, and （98.74±0.76）% respectively. In CIK cells. the proportions of CD3+，CD3+CD4+，CD3+CD8+ and CD3+CD16+/CD56+ cells were （91.98±5.9）%，（25.19±10.5）%，（71.15±7.8）% and （18.73±7.4）% respectively. The median OS was 77 months in the DC-CIK treatment group and compared to OS median of 51 months in the control group, the difference was significant (χ2＝4.431，P<0.05). DC-CIK therapy, adjuvant chemotherapy and TNM stage are independent prognostic factors for gastric cancer. Common adverse reactions are fever, chill and weakness in the DC-CIK therapy. Conclusion: Autologous tumor antigen-loaded DC-CIK therapy could prolong OS of patients with gastric cancer after operation of gastric cancer, and have little adverse reactions as well as be safety and effective.

国家临床重点专科建设资助项目；福建省临床重点专科建设资助项目；福建省医学创新课题资助项目（No. 2015-CXB-4）