目的：探讨沉默叉头框蛋白Q1(forkhead box Q1，FOXQ1)基因后对肝细胞癌（hepatocellular carcinoma，HCC）SMMC-7721细胞迁移侵袭能力的影响及其机制。方法: 制备FOXQ1-shRNA、NC-shRNA重组慢病毒，将其感染到SMMC-7721细胞中；实验设干扰组、阴性对照组和空白组，用Transwell小室法检测细胞迁移、侵袭能力；利用qRT-PCR和Western blotting法检测SMMC-7721细胞中FOXQ1、MMP-2和MMP-9mRNA和蛋白的表达。结果: 随着肝癌SMMC-7721细胞的迁移侵袭能力的增强，FOXQ1的表达逐渐增加；干扰组较阴性对照组与空白组FOXQ1表达水平降低\[（0.34±0.03）vs（0.89±007）和（0.84±0.05），P<0.05\]；沉默FOXQ1基因后，SMMC-7721细胞的迁移侵袭能力显著下降\[(9.67±1.15) vs （25.67±208）和(27.33±2.52)，P<0.05\]，MMP-2与MMP-9表达水平下降\[MMP-2：(0.35±0.04) vs (0.61±0.05)和(065±008)；MMP-9： (0.40±0.05) vs (0.73±0.07)和(0.77±0.06)，均P<0.05\]。结论: 沉默FOXQ1基因的表达能够抑制肝癌SMMC-7721细胞的迁移、侵袭能力，其机制可能与MMP-2与MMP-9的表达下调有关。

Objective:To explore the effect and mechanism of Forkhead box Q1 (FOXQ1) gene silencing on migration and invasion abilities of hepatocellular carcinoma cell line SMMC-7721. Methods: FOXQ1-shRNA and NC-shRNA recombinant lentiviral vectors were constructed and transduced into SMMC-7721 cells. There were interfered group, negative group and blank group. Transwell chamber assay was used to detect the abilities of cell migration and invasion. qRT-PCR and Western blotting were used to examine the mRNA and protein expressions of FOXQ1, MMP-2 and MMP-9 in SMMC-7721 cells.Results: FOXQ1 expression level was positively correlated to the migration and invasion abilities of SMMC-7721 cells; Compared with the negative group and blank group, the expression level of FOXQ1 was decreased in interfered group (\[0.34±0.03\] vs \[0.89±0.07\] and \[0.84±0.05\], P<0.05); After FOXQ1gene silencing, the cell migration and invasion abilities of SMMC-7721 cells were significantly decreased (\[9.67±1.15\] vs \[25.67±2.08\] and \[2733±252\]，P<0.05\], in the meanwhile, the expressions of MMP-2 and MMP-9 were down-regulated significantly \[MMP-2: \[0.35±0.04\] vs \[0.61±0.05\] and \[0.65±0.08\]；MMP-9: \[0.40±0.05\] vs \[0.73±0.07\] and \[0.77±006\], all P<0.05). Conclusion: FOXQ1 gene silencing could suppress migration and invasion abilities of hepatocellular carcinoma cell line SMMC-7721. Its mechanism might be related to the down-regulation of MMP-2 and MMP-9.

四川省教育厅科研资助项目（No.12ZB218）