目的：探讨木鳖子单体化合物对羟基桂皮醛（p-hydroxylcinnamaldehyde，CMSP）对食管癌细胞增殖及在BALB/c裸鼠移植瘤生长的抑制作用。方法： 细胞计数法检测CMSP对食管癌细胞株Kyse 30和TE-13增殖的抑制作用，RT-PCR法检测食管癌细胞中CEA和SCC mRNA的表达情况，Western blotting法检测食管癌细胞中C-myc和N-myc蛋白的表达变化。构建裸鼠食管癌细胞移植瘤模型，分为对照组和CMSP处理组，实验结束后测量移植瘤大小及体积；免疫组织化学SP法观察移植瘤组织C-myc和N-myc蛋白的表达变化，H-E染色后光镜下观察两组裸鼠移植瘤组织和肝、脾组织的病理学变化。结果：CMSP对食管癌细胞Kyse 30和TE-13的增殖具有明显的抑制作用\[（1.7±0.3) vs (3.8±0.3)，(1.6±0.2) vs (4.5±0.4)，均P<001\]。经CMSP处理后：（1）Kyse 30和TE-13细胞中CEA和SCC mRNA表达降低（P<0.01)、C-myc和N-myc蛋白表达明显低于对照组（P<0.05或P<0.01）；（2）裸鼠移植瘤平均体积和质量明显降低（P<0.05或P<0.01），移植瘤组织中C-myc和N-myc蛋白表达水平低于盐水对照组肿瘤组织。CMSP处理组和对照组中肝、脾组织并未发生任何病理改变。结论：木鳖子单体化合物CMSP体外抑制食管癌细胞增殖，体内抑制裸鼠食管癌移植瘤的生长。

Objective: To study the inhibitory effects of p-hydroxylcinnamaldehyde（CMSP）on the proliferation of esophageal cancer cells and the growth of their xenograft tumors on BABL/c nude mouse. Methods:The inhibitory effects of CMSP on the proliferation of Kyse 30 and TE-13 esophageal cancer cells were measured by cytometry. CEA and SCC mRNA expressions in esophageal cancer cells were detected with RT-PCR assay; the expression levels of C-myc and N-myc in esophageal cancer cells were detected by Western blotting; Esophageal cancer xenograft model was constructed on nude mouse, and the mice were divided into CMSP group and control group. After experiment ended, the tumor weight and size were measured. The expression levels of C-myc and N-myc in xenografts were detected by immunohistochemical SP assay; Morphological changes of liver, spleen and tumor tissues of nude mouse were observed by H-E staining under light microscope.Results: CMSP had obvious inhibitory effect on esophageal cancer cells Kyse 30 and TE-13（\[1.7±03\] vs \[3.8±0.3\]，\[1.6±0.2\] vs \[4.5±0.4\]，P<0.01）. Compared to the control group, after CMSP treatment,(1)the expressions of CEA and SCC mRNA in Kyse 30 and TE-13 cells were inhibited (P<0.01); the expressions of C-myc and N-myc proteins were significantly inhibited (P<0.05,P<0.01); (2) the average volume and weight of xenograft tumor in CMSP group significantly decreased (P<0.05,P<001); the expressions of C-myc and N-myc proteins were decreased. No obvious morphological changes were found in liver and spleen tissues in both CMSP treatment group and control group. Conclusion: CMSP has inhibitory effects on both esophageal cancer cells in vitro and their nude mouse xenograft tumors in vivo.

国家自然科学基金青年基金资助项目（No. 81502032）；河北省医学科学研究重点课题计划资助项目（No.20120120）