目的：探讨焦脱镁叶绿酸-a甲酯介导的光动力疗法(methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT) 对人骨肉瘤MG-63细胞迁移及侵袭的影响及其可能机制。方法: 采用CCK-8检测经 MPPa-PDT 处理后不同时间点的人骨肉瘤MG-63细胞增殖活性；划痕实验及Transwell实验检测细胞迁移、侵袭能力；Western blotting检测E-钙黏蛋白（E-cad）和基质金属蛋白酶（matrix metalloproteinase，MMP）-2、-9蛋白的表达水平。结果: MPPa-PDT 处理MG-63细胞12、24及48 h后MPPa-PDT组细胞增殖能力明显较对照组、MPPa组和LED组细胞降低（均P<0.05)，且MPPa-PDT组细胞的划痕愈合能力、迁移活力及侵袭能力也较这3组细胞显著下降（均P<0.05)；MPPa-PDT组细胞E-cad的表达显著高于其他3组，MMP-2、MMP-9的表达则明显低于其他3组 (均P<0.05)。结论： MPPa-PDT抑制人骨肉瘤 MG-63细胞的侵袭和迁移能力；上调E-cad的表达，下调MMP-2、-9的表达可能是其作用机制之一。

Objective:To explore effect of methyl ester pyropheophorbide-a mediated photodynamic therapy (MPPa-PDT) on invasion and migration of human osteosarcoma MG-63 line cell and its possible mechanism. Methods: Using CCK8 assay, cytoactive of the MG-63 cell was detected at various time points of post-treatment with MMPa-PDT. Migration and invasion abilities of the MG-63 cell were respectively examined by Scratch and Transwell tests. Western blotting assay was used to check expression levels of E-cadherin (E-cad), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MM-9) proteins in the MG-63 cell. Results: After treatment with MPPa-PDT, proliferation ability of the MG-63 cell at 12, 24 and 48 h in MPPa-PDT group was significantly lower than those in control, MPPa and LED groups (all P<0.05), and scratch healing ability, migration activity and invasion ability of the MG-63 cell in the MPPa-PDT group were also obviously more decline than those in the other three groups (all P<0.05). Expression of E-cad protein in the MG-63 cell of the MPPa-PDT group was evidently higher than those in the MG-63 cell of the other three groups, while expressions of MMP-2 and MMP-9 proteins in the MG-63 cell of the MPPa-PDT group were lower than those in the MG-63 cell of the other three groups (all P<0.05). Conclusion: MPPa-PDT could inhibit invasion and migration abilities of the human osteosarcoma MG-63 line cell, and could up-regulate expression of E-cad and down-regulate expressions of MMP-2 and MMP-9，which could be one of the mechanisms responsible for the above inhibition action.

国家自然科学基金项目（No. 81572634），重庆市教育委员会研究生科研创新项目（No.CYS15141）