目的：探讨高迁移率族蛋白B1和N1（high mobility group B1 and N1,HMGB1 and HMGN1)在宫颈癌肿瘤细胞中的表达情况与肿瘤浸润淋巴细胞（tumor infiltrating lymphocytes，TILs）的关系。方法：收集2012年4月至2015年9月在天津医科大学附属肿瘤医院就诊的100例早期宫颈癌患者的术后肿瘤组织蜡块标本，其中Ⅰ和Ⅱ期各50例。免疫组织化学方法检测肿瘤组织中HMGB1、HMGN1、CD3和CD8的表达。根据总体的染色强度将HMGB1和HMGN1分别分为高表达组和低表达组；根据有无细胞质表达分别分为有胞质表达组和无胞质表达组。分别比较高、低表达组间及有、无胞质表达组间肿瘤组织内CD3+和CD8+细胞数。结果：肿瘤组织内HMGB1和HMGN1的表达强度及有、无胞质表达与患者年龄、FIGO分期、病理分级均没有明显相关性（P>0.05）;HMGB1与HMGN1两者的胞质表达呈正相关关系（P<0.05）。HMGB1和HMGN1有胞质表达组的 CD3+和CD8+细胞较无胞质表达组要高（P<0.05）。 结论：宫颈癌细胞HMGB1和HMGN1的胞质表达与肿瘤组织内高水平TILs相关，这一发现有望为宫颈癌的免疫治疗提供新的策略。

Objective:To explore expression status of high mobility group B1 and N1 (HMGB1 and HMGN1) in cervical carcinoma cells and their relationship with tumor infiltrating lymphocytes (TILs). Methods:One hundred postoperative tumor tissue specimens from the patients with early cervical carcinoma who were hospitalized in Cancer Hospital of Tianjin Medical University during April 2012 to September 2015 were collected, among them stage Ⅰ and stage Ⅱ in each of 50 cases. Immune histochemical assay was used to detect expressions of HMGB1, HMGN1, CD3 and CD8 in the tumor tissues. According to overall staining intensity, HMGB1 and HMGN1 were divided into high expression group and low expression group respectively, and divided into cytoplasmic expression group and no cytoplasmic expression group based on expression of cytoplasm. Number of CD3+ and CD8+ cells in the tumor tissues were compared between the high expression group and the low expression group as well as between cytoplasmic expression group and no cytoplasmic expression group respectively. Results: Expression intensities of HMGB1 and HMGN1 in the tumor tissues as well as whether or not expression of HMGB1 and HMGN1 in cytoplasm all did not obviously correlate with age, FIGO staging and pathological grade (all P>0.05). Expressions of HMGB1 and HMGN1 in cytoplasm showed a positive correlation (P<0.05). Numbers of CD3+ and CD8+ cells in cytoplasm with expressions of HMGB1 and HMGN1 groups were significantly higher than those in cytoplasm without expressions of HMGB1 and HMGN1 groups (P<0.05).Conclusion: Cytoplasmic expressions of HMGB1 and HMGN1 in cervical carcinoma cells could correlate with high level of TILs in the tumor tissue, and this finding could be expected to provide a novel strategy for immunotherapy of cervical carcinoma.

国家科技支撑计划(No. 2015BAI12B12)；重大新药创新科技专项子课题(No. 2015ZX09102018)；天津市抗癌重大专项攻关计划(No. 14ZCKZSY00166)；国家自然科学基金(No. 81672697)