目的：探讨长链非编码RNA (long non-coding RNA，lncRNA)肌动蛋白纤维相关蛋白-1反义RNA1（actin filament-associated protein 1-antisense RNA1, AFAP1-AS1 RNA1）在胃癌组织中的表达情况及其临床意义。 方法：采用Real-time PCR方法检测2010年1月至2014年12月宜昌市第二人民医院及三峡大学仁和医院手术切除的274例胃癌组织及相应癌旁正常胃组织中AFAP1-AS1的表达，并分析AFAP1-AS1表达量与临床及病理特征的关系。结果：AFAP1-AS1在胃癌组织比癌旁正常组织显著高表达(P<0.01)。AFAP1-AS1在早期胃癌组织中平均表达量明显低于进展期胃癌(P<0.01)。在不同病理类型胃癌中，AFAP1-AS1的表达量没有差异(P>0.05)。AFAP1-AS1表达量和胃癌淋巴侵袭或远处转移密切相关，在伴有淋巴结侵袭或远处转移的胃癌组织中，AFAP1-AS1明显高表达(P<0.01)。结论： AFAP1-AS1可能是胃癌发生发展的一个重要因素；有望成为新的胃癌预后标志物，用于胃癌的临床诊断和预后判断。

Objective:To explore expression of actin filament-associated protein 1-antisense RNA1 (AFAP1-AS1), long non-coding RNA (lncRNA), in the gastric carcinoma tissues and its clinical significance. Methods: Using real-time PCR assay, expressions of AFAP1-AS1 in 274 cases of gastric carcinoma tissues and their corresponding adjacent normal gastric tissues which collected from the patients with gastric carcinoma who were hospitalized in the 2nd People Hospital of Yichang City and Renhe Hospital of Sanxia University for operative treatment during January 2010 to December 2014 were detected. And relationship between the expression of AFAP1-AS1 and clinicopathological features of the patients with gastric carcinoma was analyzed. Results: Expression of AFAP1-AS1 in the gastric carcinoma tissue was obviously higher than that in the adjaceut normal gastric tissues. Mean expression amount of AFAP1-AS1 in the early gastric carcinoma tissue was significantly lower than that in the advanced gastric carcinoma tissue (P<0.01). Among the gastric carcinoma tissues of various pathological types, expression amounts of AFAP1-AS1 were not significantly different (P>0.05). Expression amounts of AFAP1-AS1 were closely correlated with lymphatic invasion and distant metastasis of the gastric carcinoma. Expression amounts of AFAP1-AS1 in the gastric carcinoma tissue with local invasion and distant metastasis were obviously higher than those in the gastric carcinoma tissues without local invasion and distant metastasis (P<0.05). Conclusion:AFAP1-AS1 could be an important regulatory factor in the gastric carcinogenesis and development. It could be expected to become a novel marker for judging prognosis of the gastric carcinoma and used for clinical diagnosis and prognosis evaluation of the gastric carcinoma.