目的：检测卵巢癌构域蛋白酶1（ovarian tumor domain-containing protease 1, OTUD1）在非小细胞肺癌(non-small cell lung cancer,NSCLC) 组织中的表达水平，探讨OTUD1表达与NSCLC临床病理特征及预后的关系。方法:选取2008年3月至2009年2月在山东大学齐鲁医院胸外科行手术治疗的82例非小细胞肺癌患者组织标本及40例正常肺组织标本，免疫组化法检测OTUD1的表达水平；另取2015年3月至2015年4月在山东大学齐鲁医院胸外科手术治疗的原发性NSCLC 25例，应用Real-time PCR 检测OTUD1在 NSCLC 组织及邻近正常肺组织内的表达。结果: 与正常肺组织相比，NSCLC组织中OTUD1mRNA的表达显著增高（0.620±0.055 vs 0.387±0.037, P<0.01）；OTUD1的表达与患者的年龄、性别、吸烟史、病理类型均无显著相关性（均P>0.05）；与淋巴结转移(P<0.01)、分化程度(P<0.01)及TNM分期(P<0.05)显著相关。单因素及多因素分析结果显示，OTUD1高表达与患者不良预后有关。结论: OTUD1在NSCLC组织中高表达，与NSCL的恶性进展及不良预后密切相关，可作为判断NSCLC生物学特征及预后的参考指标。

Objective:To detect expression level of ovarian tumor domain-containing protease 1 (OTUD1) in the tissue of non-small cell lung cancer (NSCLC) and to explore relationship between the expression of OTUD1 and clinical pathological features and prognosis of the patients with NSCLC. Methods: Cancer tissues from 82 patients with NSCLC, who were hospitalized in Department of Thoracic Surgery, Qilu Hospital of Shandong University during March 2008 to February 2009 for operative treatment and 40 specimens of normal lung tissues were selected, and expression levels of OTUD1 were detected by immune histochemical method. And another 25 patients with primary NSCLC who were hospitalized in Department of Thoracic Surgery, Qilu Hospital of Shandong University during March to April 2015 for operative treatment were collected and expressions of OTUD1in the NSCLC tissues and their adjacent normal lung tissues were examined by Real-time PCR assay. Results: Comparing with the normal lung tissues, expressions of OTUD1 mRNA in NSCLC tissues were distinct higher （0.620±0055 vs 0.387±0.037, P<0.01）. Expression of OTUD1 did not significantly correlated with age, gender, smoking history and pathologic type of the patients with NSCLC (all P>0.05), and significantly correlated with lymph node metastasis, differentiation grads and TNM staging of the patiewts with NSCLC (P<0.05 or P<0.01). Results of univariate and multivariate analysis showed that high expression of OTUD1 associated with poor prognosis of the patients. Conclusion: High expression of OTUD1 in the NSCLC tissues could be closely related to malignant progression and poor prognosis of NSCLC. Expression of OTUD1 could be as a reference index to evaluate biological features of NSCLC and prognosis of the patients with NSCLC.

国家自然科学基金资助项目( No.30571844 )；山东省自然科学基金资助项目( No.ZR2013HM089)；吴阶平医学基金资助项目(No.320.6750.12393)