目的：探讨针对趋化因子受体基因 CXCR4和CXCR7的小干扰RNA(siRNA)对人子宫内膜癌Ishikawa细胞裸鼠移植瘤生长的影响。方法：将子宫内膜癌Ishikawa细胞株注射于裸鼠肩胛背部皮下建立动物模型，待肿瘤最长径达5~7 mm后，将所有荷瘤裸鼠随机分组，分别给予CXCR4-siRNA和CXCR7-siRNA单独或联合瘤体内注射，并以阴性对照siRNA和生理盐水作为对照组，每3 d治疗一次，共6个治疗周期。观察各组裸鼠移植瘤的生长，比较各组移植瘤的体积和质量的差异，并以实时荧光定量RT-PCR、Western blotting和免疫组化技术验证CXCR4和CXCR7的基因沉默效果。结果： 成功建立Ishikawa细胞裸鼠移植瘤模型，与阴性对照siRNA组和空白对照组相比，CXCR4 siRNA治疗组、CXCR-siRNA治疗组和CXCR4-siRNA+CXCR7-siRNA治疗组的肿瘤的生长均明显受到抑制（均P<0.05）；CXCR4-siRNA和/或CXCR7-siRNA瘤体内直接注射能显著下调CXCR4、CXCR7mRNA（均P＜0.05）和蛋白（均P＜0.01）的表达。结论： siRNA干扰CXCR4和CXCR7表达能够有效抑制人子宫内膜癌Ishikawa细胞裸鼠移植瘤的生长。

Objective:To evaluate the effect of small interfering RNA (siRNA) of CXCR4 and CXCR7 on the growth of endometrial cancer (EC) Ishikawa cells xenografts in nude mice. Methods: EC Ishikawa cells were subcutaneously injected into the right flank of nude mice to establish animal xenograft model. When the diameter of tumors reached 5-7 mm, all tumor-bearing mice were randomly divided into five groups, and treated with intra-tumor injection of CXCR4-siRNA, CXCR7-siRNA, CXCR4-siRNA+CXCR7-siRNA, negative-siRNA, and normal saline, respectively. Growth of xenografts was observed, and the differences in volume and weight of xenografts among five groups were examined. CXCR4 and CXCR7expressions in tumor xenografts were detected by quantitative reverse transcription polymerase chain reaction (QRT-PCR), Western blotting and immunohistochemical technique, respectively. Results: Ishikawa cells xenograft model in nude mice was successfully constructed. The growth of tumor xenografts in CXCR4 siRNA group, CXCR-siRNA group and CXCR4-siRNA+CXCR7-siRNA group were significantly inhibited, compared with those in negative control and blank control group (all P<0.05). The mRNA (P<0.05) and protein (P<0.01) expressions of CXCR4 and CXCR7 were statistically down-regulated by directly injecting siRNA into xenografts. Conclusion: siRNA interfering CXCR4and CXCR7could effectively suppress the growth of human EC Ishikawa xenografts in nude mice.

山东省优秀中青年科学家科研奖励基金资助项目（No.BS2009SW002），山东省自然科学基金资助项目（No.ZR2013HM012）