目的：探讨青蒿琥酯（artesunate，ART）对人肺癌细胞株A549增殖、迁移和侵袭的影响及其可能机制。方法：采用CCK-8法检测不同质量浓度的ART对A549细胞作用24、48 h后细胞的增殖水平，Transwell小室试验检测30 μg/ml青蒿琥酯对A549细胞侵袭能力的影响，划痕实验检测对A549细胞迁移能力的影响。Western blotting检测A549细胞经30 μg/ml的ART作用48 h后人抗原R（human antigen R, HuR）和MMP-9蛋白表达变化。结果： ART能够抑制人肺癌A549细胞的增殖，呈剂量依赖性（P<0.05）。与未处理组比较，ART（30 μg/ml）处理的A549细胞穿膜的细胞数目显著减少\[（47.11±861） vs （131.00±14.58）个，P<0.01\]；A549细胞迁移距离显著缩短\[（1.08±0.13) vs （2.91±0.24）mm，P<0.01\]；A549细胞内HuR和MMP-9蛋白表达水平降低（P<0.05）。结论：ART能够抑制肺癌A549细胞的增殖、迁移和侵袭，其机制可能与抑制HuR的表达有关。

Objective: To investigate the effects of artesunate (ART) on the proliferation, invasion and migratory ability of human lung cancer A549 cells and its possible mechanisms. Methods: CCK-8 assay was used to analyze the proliferation level of A549 cells after treatment with different concentrations of ART for 24 and 48 h. Transwell assay and wound healing assay were adopted to evaluate the invasion and migration of A549 cells after the treatment of ART at concentration of 30 μg/ml, respectively. Western blotting was used to detect the change in protein expressions of human antigen R (HuR) and MMP-9 in A549 cells after the treatment with ART (30 μg/ml). Results:ART inhibited the proliferation of human lung cancer A549 cells in a dose-dependent manner (P<0.05). Compared with untreated group, number of A549 cells penetrating the membrane in ART group (30μg/ml) was significantly decreased(\[47.11±8.61\] vs \[131.00±1458\], P<001), and the migration distance was remarkably reduced (\[1.08±0.13\] vs \[2.91±0.24\] mm, P<001). In addition, ART significantly inhibited the protein expression of HuR and MMP-9 in A549 cells (P<0.05). Conclusion: ART can inhibit the proliferation, invasion and migration of A549 cells, and this effect may be related to inhibition of HuR expression.

国家自然科学基金资助项目(No.81572875,No.81272619)