目的：探讨染色质解旋酶DNA结合蛋白1样基因（chromodomain helicase/ATPase DNA binding protein 1-like gene, CHD1L）对前列腺癌细胞侵袭、迁移能力的影响及其可能的作用机制。方法：采用实时荧光定量PCR技术检测前列腺癌细胞株LNCAP、PC3、DU145以及前列腺上皮细胞株RWPE-1中CHD1L mRNA表达水平；转染siRNA干扰前列腺癌PC3细胞CHD1L的表达，并用Transwell侵袭实验和划痕实验分析沉默CHD1L对前列腺癌细胞侵袭和迁移能力的影响；Western blotting检测PC3细胞MMP-9、N-钙黏蛋白和E-钙黏蛋白的表达水平。结果: CHD1LmRNA在前列腺癌细胞中的表达水平明显高于前列腺上皮细胞（P<0.01），其中以前列腺癌PC3细胞的表达水平最高。侵袭实验中，干扰组的穿膜细胞数明显低于阴性对照组和空白对照组\[（49.67±6.67） vs （113.67±5.69）和（112.00±12.49）个，P<0.05）。划痕实验中，干扰组48 h伤口愈合率也低于阴性对照组和空白对照组\[（21.27±3.27）% vs（48.47±5.72）%和（49.93±3.35）%，P<0.05\]。干扰组细胞MMP-9和N-钙黏蛋白表达下调，E-钙黏蛋白表达上调。结论: 沉默CHD1L可降低前列腺癌PC3细胞的侵袭迁移能力，该作用可能是通过调控MMP-9和EMT相关蛋白表达实现的。

Objective:To explore the influence of CHD1L gene (chromodomain helicase/ATPase DNA binding protein 1-like gene) on the invasion and migration ability of prostate cancer cells and the potential mechanisms. Methods: Real-time fluorescent quantitative-PCR assay was carried out to detect mRNA expression of CHD1L gene in prostate cancer cell lines LNCAP, PC3 and DU 145 as well as normal prostate epithelial cell line RWPE-1. siRNA transfection was used to deplete CHD1L expression in PC3 cells. Then Transwell invasion assay and wound healing test were employed to detect the effect of CHD1Lsilencing on the invasion and migration ability of PC3 cells. Protein expressions of MMP-9, N-cadherin and E-cadherin were examined by Western blotting assay. Results: Compared to normal prostate epithelial cells, three prostate cancer cell lines all showed significantly higher expression of CHD1L mRNA (P<0.01), and PC3 cells demonstrated the highest CHD1LmRNA expression. Invasion assay showed that the trans-membrane cell number in CHD1L silencing group was significantly lower than that in negative and blank control groups (49.67±6.67 vs 113.67±5.69 and 112.00±12.49, P<0.05). Wound healing test also exhibited lower healing degree in CHD1L silencing group than that in negative and blank control groups at 48 h (\[21.27±3.27\]% vs \[48.47±5.72\]% and \[49.93±3.35\]%, respectively, P<0.05). Furthermore, protein expressions of MMP-9 and N-cadherin decreased while E-cadherin increased in CHD1L gene depletion cells. Conclusions: CHD1L silencing could dampen the invasion and migration ability of prostate cancer PC3 cell line, and this might be accomplished by regulating MMP-9 and EMT related proteins.

河南省医学科技攻关计划项目（No. 201303155）