目的：探讨人胎盘提取物（human placenta extracts，HPE）对人结肠癌细胞株Caco-2及SW480增殖和迁移能力的影响。方法：用不同质量浓度HPE处理Caco-2和SW480细胞，CCK-8、Ki-67、CFSE法检测细胞增殖活性的变化，Annexin-V/PI及DAPI细胞核染色检测HPE对细胞周期及凋亡的影响，实时荧光定量PCR法检测HPE对细胞中BAX、CDK2及CyclinA2基因表达的影响，划痕实验检测对细胞迁移能力的影响。建立裸鼠结肠癌移植瘤模型，随机分组分别单独或联合给予HPE和5-氟尿嘧啶(5-Fu)，观察裸鼠移植瘤的生长状况。结果：HPE处理组Caco-2及SW480细胞增殖能力低于对照组\[（0.82±001） vs (0.96±0.02)，P<0.01；(0.90±0.03) vs (0.96±0.02)，P<0.05\]，细胞凋亡率高于对照组\[(20.47±1.32)% vs (11.01±382)%，P<0.01；(20.70±5.19)% vs (8.00±2.69)%，P<0.05\]；HPE处理组细胞胞质减少、核固缩、BAX基因表达增加\[（323±1.90) vs (1.00±0.00)，(2.25±0.55) vs (1.00±0.00)，均P<0.05\]；细胞停留在细胞DNA复制S期，出现凋亡峰；Caco-2细胞CDK2及CyclinA2基因表达降低（P<0.05）；并且细胞的迁移能力低于对照组\[(0.17±0.29) vs （1.50±050)mm，P<0.05\]。HPE处理组裸鼠移植瘤体积小于对照组、生存率高于单用5-Fu组（P<0.05）。结论: HPE在体内外通过干预结肠癌细胞凋亡、阻滞细胞周期、抑制细胞增殖及迁移，可能对抑制肿瘤细胞增殖有一定作用。

Objective:To explore the effect of human placenta extracts (HPE) on the proliferation and migration of human colon cancer cell lines (Caco-2 and SW480). Methods: Human colon cancer Caco-2 and SW480 cell lines were treated with different concentrations of HPE. Cell proliferation was determined by CCK-8, Ki-67 and CFSE dilution. The effect of HPE on cell apoptosis and cell cycle were detected by Annexin-V/PI and DAPI staining. The expressions of apoptosis related gene BAX, CDK2and CyclinA2 were determined by Real-time PCR. The migration efficiency of cell was measured by wound-healing assay. Human colon cancer cells were subcutaneously injected into the nude mice to establish xenograft model, and the mice were randomly divided into different groups and given different treatments of HPE and/or 5-fluorouracil (5-Fu). The tumor volume and body weight were recorded. Results: The cell proliferation ability of both Caco-2 and SW480 cells cultured with HPE was lower than that of control group (0.82± 0.01 vs 0.96±0.02, P<001; 0.90±0.03 vs 0.96±0.02, P<0.05); and the apoptotic rates in HPE treatment group were significantly higher than control group (\[20.47±1.32\]% vs \[11.01±3.82\]%, P<0.01; \[20.70±5.19\]% vs \[8.00±2.69\]%, P<005) , accompanied with cytoplasmic reduction, nuclear pyknosis and increased BAX gene expression (3.23±1.90 vs 1.00±0.00; 2.25±0.55 vs 1.00±0.00, all P<0.05). Furthermore, S-phase arrest with an apoptosis peak was observed in HPE treatment group, and decreased mRNA expressions of CDK2 and CyclinA2 (all P<0.05) in Caco-2 cells were also observed. Moreover, an obvious lower wound-healing rate was identified in cells treated with HPE (\[0.17±029\] vs \[1.50±0.50\] mm, P<0.05). The tumor volume of mice in HPE-treated group was smaller than that of control group, and higher survival rate was observed than that of 5-Fu alone group. Conclusion: This study indicated that HPE could inhibit the proliferation and migration ability of human colon cancer cells both in vitro and in vivo, partly through enhancing cell apoptosis and S-phase arrest, which may contribute to the inhibition on growth of certain tumor cells.

国家自然科学基金资助项目（No.81560266）；贵州医科大学附属医院博士启动基金资助项目。