目的：探讨表达IL-29的重组新城疫病毒（recombinant Newcastle disease virus IL-29，rNDV IL-29）对肺腺癌A549细胞增殖、迁移和细胞凋亡的影响。方法：实验分为rNDV IL-29组（rNDV IL-29感染A549细胞）、NDV组（NDV感染A549细胞）和对照组（PBS）。用Western blotting、RT-PCR、免疫荧光法检测IL-29蛋白及其mRNA的表达；MTT、克隆形成实验及划痕实验检测rNDV IL-29对A549细胞增殖、迁移能力的影响；流式细胞术及Western blotting检测rNDV IL-29对A549细胞凋亡的影响。结果：与NDV组和对照组相比，rNDV IL-29组细胞：(1) IL-29蛋白及其mRNA表达水平显著升高；（2）细胞增殖抑制率明显升高\[（56.48±1189）% vs （42.18±6.18）%, P<0.05\]，细胞迁移率明显下降\[（5.00±4.00）% vs （32.43±3.71）%、（84.57±396）%, 均P<0.05\]；（3）Caspase3 表达水平明显上调\[（29.67±1.53）% vs （18.33±3.06）%、（7.00±6.08）%, 均P<0.05\]，Bcl-2/bax表达水平明显下调\[（12.00±2.65）% vs （42.33±5.86）%、（67.00±6.25）%, 均P<0.05\]，细胞凋亡增多。结论：rNDV IL-29抑制肺腺癌A549细胞增殖、迁移，并且促进其凋亡，提示rNDV IL-29可能成为一种很有潜力的抗癌药物。

Objective: To investigate the effects of recombinant Newcastle disease virus (rNDV) expressing IL-29（rNDV IL-29）on the proliferation, migration and apoptosis of lung adenocarcinoma A549 cells. Methods: A549 cells were treated with rNDV IL-29, NDV and PBS respectively, namely rNDV IL-29 group, NDV group and control group. The protein and gene expression of IL-29 in A549 cells were examined by RT-PCR, immunofluorescence and Western blotting; MTT assay, colony formation assay and scratch assay were used to evaluate the effect of rNDV IL-29 on proliferation and migration ability of A549 cells; Flow cytometry and Western blotting were used to examine the effect of rNDV IL-29 on apoptosis of A549 cells. Results:Compared with NDV group and control group, (1) IL-29 protein and gene expressions were dramatically increased in rNDV IL-29 group. (2) the rate of cell proliferation in rNDV IL-29 group was significantly inhibited (\[56.48±11.89\]% vs \[42.18±6.18\]%, P<0.05), and migration ability of A549 cells in rNDV IL-29 group was statistically decreased (\[5.00±4.00\]% vs \[32.43±3.71\]%, \[84.57±3.96\]%, all P<0.05). (3) the expression of caspase3 in rNDV IL-29 group was significantly up-regulated (\[29.67±1.53\]% vs \[18.33±3.06\]%, \[7.0±6.08\]%, all P<0.05), while Bcl-2/bax level was remarkably reduced (\[12.00±2.65\]% vs \[42.33±586\]%, \[67.00±6.25\]%, all P<0.05\]. Conclusion: rNDV IL-29 inhibits the proliferation and migration of A549 cells and promotes apoptosis of A549, suggesting that rNDV IL-29 may be served as a potential anticancer drug.

江苏省自然科学基金资助项目(No.BK20151333)