目的：探讨Klotho基因对人子宫颈鳞癌细胞株SiHa生物学特性的影响及其作用机制。方法：实时荧光定量PCR及Western blotting检测Klotho基因在子宫颈鳞癌细胞系SiHa、HeLa和C33A及人永生化表皮细胞HaCaT细胞中的表达水平。构建Klotho过表达载体并转染SiHa细胞，采用CCK-8法、Transwell侵袭实验和流式细胞术检测SiHa细胞增殖、凋亡和迁移能力的变化；Western blotting检测Rho A和ROCK 1蛋白表达水平。结果：Klotho在子宫颈癌SiHa、Hela、C33A细胞中表达水平低于HaCaT细胞（P<0.05）。在SiHa细胞中过表达Klotho后，（1）细胞的增殖能力显著低于对照组\[（67.37±5.04）% vs（100.34±7.62）%, P<0.05）\]；（2）G0/G1期细胞百分率显著增加\[（82.56±3.89）% vs（61.37 ±3.28）%, P<0.05\]、S期细胞百分率显著减少\[（9.12±2.48）% vs（28.97 ± 2.08）%, P<0.01\]；（3）细胞早期凋亡率和晚期凋亡率均显著增加（均P<0.05）；（4）细胞迁移率显著降低（P<0.01）；（5）Rho A和ROCK 1蛋白表达水平均显著下降（均P<0.01）。结论：Klotho能够抑制人子宫颈癌SiHa细胞的增殖和迁移，并促进其凋亡；作用机制可能与抑制Rho A/ROCK 1信号通路的活化有关，提示Klotho可以作为诊断和靶向治疗子宫颈癌的潜在作用位点。

Objective:To investigate the effect and mechanism of Klotho gene on the biological characteristics of cervical squamous cell carcinoma SiHa cells. Methods: The expression of Klotho in the cervical squamous cell carcinoma cell SiHa, HeLa and C33A lines and the human immortalized epidermal cell HaCaT line was detected by Real-time PCR and Western blotting. Vectors over-expressing Klotho were constructed and used to transfect SiHa cells, and then the cell proliferation, apoptosis and migration were detected with CCK-8 assay, Flow cytometry and Transwell assay, respectively. The expressions of Rho A and ROCK 1 at protein level were measured with Western blotting assay. Results: Expressions of Klotho in cervical cancer cell SiHa, Hela and C33A lines were lower than that in the HeCaT line (P<0.05). After over-expression of Klotho in SiHa cells, (1) cell proliferation was significantly decreased, compared with control group (\[67.37±5.04\]% vs \[100.34±7.62\]%, P<0.05); (2) the percentage of cells at G0/G1 phrase was increased(\[61.37±3.28\]% vs \[82.56±3.89\]%, P<0.05), while the percentage at S phrase was decreased (\[9.12±248\]% vs \[28.97±2.08\]%, P<0.01); (3) cell apoptotic rate at both early and late stage were all decreased (all P<0.05); (4) cell migration was suppressed (P<0.01); (5) the expressions of Rho A and ROCK 1 at protein level were significantly decreased (all P<0.01). Conclusion: Klotho suppressed SiHa cell proliferation and migration, and promoted cell apoptosis; the mechanism may be related to the suppression of Rho A/ROCK 1 signaling pathway activation, suggesting that Klotho may be used as a potential new target for diagnosis and targeted therapy of cervical squamous cell carcinoma.