目的：探讨miR-200c在胃癌组织中的表达水平与胃癌患者临床病理特征及无病生存期（diease free survial，DFS）的关系。方法：〖HT5"SS〗 收集河北医科大学第四医院普外科2012年5月至2013年1月64例胃癌手术切除的标本及相关临床资料，采用实时荧光定量PCR技术检测miR-200c在胃癌组织和配对癌旁非癌组织中的表达。回顾性分析miR-200c表达水平与胃癌患者的临床病理特征及DFS相关性。结果：胃癌组织中miR-200c的表达水平显著低于癌旁非癌组织（3.29 vs 5.91，P<0.01）。miR-200c的表达水平与肿瘤TNM分期、浸润深度、转移和脉管瘤栓呈显著负相关（均P<0.01）。miR-200c高表达组患者中位DFS明显长于低表达组患者(22.0 vs 13.5个月，P<0.01)，其表达水平与患者DFS呈正相关（P<0.01）。结论： miR-200c在胃癌组织中低表达，其表达水平与肿瘤TNM分期、肿瘤浸润深度和脉管瘤栓呈负相关，与DFS呈正相关，在胃癌的发生发展及预后中具有重要作用。

Objective:To investigate the relationship between miR-200c expression and the clinical pathologic characteristics and disease free survival (DFS) of gastric cancer patients. Methods: Sixty-four gastric cancer samples resected from May 2012 to January 2016 at General Surgery Department of the Fourth Hospital of Hebei Medical University were collected in this study, and the relative clinical data were retrospectively analyzed. Real-time fluorescence quantification PCR was adopted to detect the expression of miR-200c in the gastric cancer tissues and corresponding para-cancerous tissues. The correlation between miR-200c expression and clinicopathological features as well as DFS was retrospectively analyzed. Results: miR-200c was dramatically decreased in gastric cancer tissues when compared to the para-cancerous tissues (329 vs 5.91, P<0.01). miR-200c expression was negatively correlated with TNM stage, tumor infiltration depth (T), metastasis(M), venous tumor thrombus (; all P<0.01). The median DFS in high miR-200c expression group was significantly longer than that of low expression group (22.0 vs 13.5 months, P<0.01), indicating a positive correlation between miR-200c expression and DFS (r=0.54, P<0.01). Conclusion:miR-200c dramatically decreased in gastric cancer tissues. Its expression level was negatively correlated with TNM stage, tumor infiltration depth and venous tumor thrombus, but positively correlated with DFS. These data suggest it may play a protective role in progress and prognosis of gastric cancer.

河北省卫生厅医学科学研究重点课题资助项目（No.20170150）