目的：研究人参皂苷Rg3通过促进乳腺癌MDAMB231细胞人乳腺珠蛋白A（mammaglobinA，MGBA）的表达从而抑制细胞增殖的作用机制。方法:MTT法和流式细胞术检测5、10、15 μg/ml Rg3对乳腺癌细胞增殖和凋亡的影响，Western blotting检测对乳腺癌细胞内MGBA表达的影响; Rg3和siRNAMGBA单独或联合处理MDAMB231细胞，MTT法和流式细胞术检测其对细胞增殖和凋亡的影响，Western blotting检测其对细胞MGBA表达的影响，敏感硫电极法检测其对乳腺癌MDAMB231细胞H2S分泌的影响。结果：作用细胞48 h后，与对照组相比，5、10、15 μg/ml Rg3 组MDAMB231细胞增殖抑制率明显增高\[（18.78±0.82）%、（33.25±1.17）%、（35.11±0.94）% vs （9.72±0.91）% , 均P<0.05\]，Rg3可促进MDAMB231细胞的凋亡（P<0.05），也明显增强MDAMB231细胞内MGBA蛋白的表达（P<0.05）。 与对照组相比，Rg3组MDAMB231细胞增殖抑制率明显升高\[（30.12±1.01）% vs（10.66±0.59）%, P<0.05\]，Rg3+siRNAMGBA组和siRNAMGBA组MDAMB231细胞增殖抑制率明显降低\[（6.61±0.63）%、（7.02±0.46）% vs （10.66±0.59）%，均P<0.05\]； Rg3组MGBA和胱硫醚γ裂解酶（cystathionineγ lyase，CSE）的表达和H2S的分泌明显增强，而Rg3+siRNAMGBA组、siRNAMGBA组明显抑制（均P<0.05）。结论：Rg3可以明显抑制乳腺癌MDAMB231细胞的增殖，并促进凋亡，其作用机制可能是通过增强MGBA蛋白的表达并激活H2S/CSE系统得以实现的。

Objective:To study the mechanism of ginsenoside Rg3 inhibiting the proliferation of breast cancer MDAMB231 cells via regulation of mammaglobinA (MGBA) expression. Methods: MTT assay and flow cytometry was used to detect the effect of different concentrations (5,10,15 μg/ml) of Rg3 on the proliferation and apoptosis of breast cancer cells; Western blotting was used to detect the expression of MGBA in breast cancer cells. MDAMB231 cells were treated with Rg3 and/or siRNAMGBA; MTT and Flow cytometry were used to detect their effect on the proliferation and apoptosis of treated cells while Western blotting was used to detect the expression of MGBA in cells; sulfur electrode assay was used to detect H2S secretion in breast cancer MDAMB231 cells. Results:48 h after cell treatment, the inhibitory rates on proliferation of MDAMB231 cells in Rg3 groups (5,10,15 μg/ml) were significantly higher than that of control group (\[18.78±0.82\]%, \[33.25±1.17\]%, \[35.11±0.94\]% vs \[9.72±0.91\]%, all P<0.05\]; Rg3 promoted the apoptosis of MDAMB231 cells (P<0.05), and significantly enhanced the expression of MGBA protein in MDAMB231 cells (P<0.05); Compared with the control group, the inhibitory rate on proliferation of MDAMB231 cells in Rg3 group was significantly increased (\[30.12±1.01)% vs (10.66±0.59)%, P<0.05\], while the inhibitory rates in Rg3+siRNAMGBA and siRNAMGBA group were significantly reduced (\[6.61±0.63\]%, \[702±0.46\]% vs \[1066±0.59\]%, all P<0.05); The expression of MGBA and cystathionineγ lyase (CSE) as well as the secretion of H2S in Rg3 group were significantly enhanced; however, their expressions in Rg3 + siRNAMGBA group and siRNAMGBA group were significantly inhibited（all P<0.05）. Conclusion: Rg3 can significantly inhibit the growth of MDAMB231 breast cancer cells and promote its apoptosis, and this effect may be realized by enhancing MGBA expression and activating H2S/CSE system.

辽宁省自然科学基金资助项目（No.2014022044）