目的：探讨lncRNA AC007009.1在非小细胞肺癌（nonsmall cell lung cancer，NSCLC）组织中的表达及其临床意义。方法：收集2011年6月至2016年6月四川省自贡市第四人民医院105例NSCLC患者；通过实时荧光定量PCR法检测lncRNA AC007009.1在105例NSCLC组织、72例癌旁组织及33正常肺组织中的表达水平，分析lncRNA AC007009.1的表达与患者部分临床病理学特征之间的关系，了解lncRNA AC007009.1的表达与患者OS和PFS关系及对患者预后的影响。结果：LncRNA AC007009.1在105例NSCLC癌组织中的表达水平明显高于癌旁组织及正常肺组织（5.30±1.96 vs 1.01±0.33、0.99± 0.29，均P＜0.01），LncRNA AC007009.1的表达水平与患者癌症分期、远处转移、病理类型及生存状态明显有关(P<0.05)。高表达lncRNA AC007009.1患者的OS及PFS较低表达患者明显缩短(P<0.01)，Cox多因素回归模型分析显示,lncRNA AC007009.1的表达水平、淋巴远处转移及临床分期是NSCLC独立的预后影响因素(P<0.05)。结论： lncRNA AC007009.1参与调节NSCLC的发生发展，可作为潜在的NSCLC诊断和预后评估的分子标志物。

Objective:To investigate the expression and clinical significance of lncRNA AC007009.1 in tissue samples of nonsmall cell lung cancer (NSCLC). Methods: The expression of lncRNA AC007009.1 in 105 NSCLC tissue samples, 72 adjacent normal tissue samples and 33 normal tissue samples was detected by qRTPCR method; and the relationship between lncRNA AC007009.1 expression and clinical pathological characteristics of NSCLC was analyzed; in addition, the correlation between lncRNAAC007009.1 expression and the OS and PFS of NSCLC patients was also analyzed. Results: Expression of lncRNAAC007009.1 was significantly increased in tumor tissues than that in noncancerous tissues (5.30±1.96 vs 1.01±0.33, 0.99±0.29，P<0.01). AC007009.1 expression was positively correlated with disease stage，distant metastasis，pathological type and survival status (P<0.05); Moreover, the OS and PFS of patients with high lncRNAAC007009.1 expression were significantly shorter than the patients with low expression (P<0.01). Cox multivariate analysis also indicated that lncRNAAC007009.1 expression, disease stage, distant metastasis were the independent prognostic markers for NSCLC (all P<0.05). Conclusion: LncRNA AC007009.1 is involved in the development of NSCLC, and can be used as a molecular marker for the diagnosis and prognosis of nonsmall cell lung cancer.

国家自然科学基金项目（No.81301910)