目的：探讨microRNA-150 （miR-150）对NK/T细胞淋巴瘤组织和NK-92细胞辐射敏感性的影响。 方法： 选取2010年1月至2016年1月在南方医科大学珠江医院收治的NK/T细胞淋巴瘤患者36例为研究对象,并收集患者的组织标本。所有患者均接受放疗并采用淋巴瘤国际工作组标准（(IWG）评价患者的近期疗效，根据疗效分为完全缓解（CR）组和未完全缓解组（Non-CR）。实时荧光定量PCR检测患者肿瘤组织及NK/T细胞淋巴瘤细胞系中miR-150的表达量，向淋巴瘤NK-92细胞转染miR-150 mimics，利用MTT法和集落形成实验分析过表达miR-150 对NK-92细胞辐射敏感性的影响，流式细胞术检测转染过表达miR-150对NK-92细胞辐射致凋亡的影响，Western blotting实验检测miR-150对凋亡相关蛋白Caspase3和PARP表达的影响。 结果： 根据IWG标准，12例患者CR，24例患者为Non-CR；与CR组相比，Non-CR组患者miR-150表达量普遍下调（ P <0.05）。与正常对照sCD3-CD56 + NK 细胞相比，NK/T细胞淋巴瘤组织（9.10±0.19 vs 4.01±0.22, P <0.01）和5种NK/T细胞淋巴瘤细胞系中miR-150呈明显低表达（ P <0.05）。过表达miR-150可明显降低辐射后NK-92细胞的增殖能力（ P <0.01）和集落形成能力（ P <0.01），明显提高NK-92细胞的辐射增敏比（10 Gy时辐增敏比为5.375)，显著促进辐射诱导的NK-92细胞的凋亡[ （37.3±1.24）% vs （28.3±2.34）%， P <0.05]，可促进激活的Caspase 3和 PARP蛋白表达。 结论： miR-150在NK/T细胞淋巴瘤组织标本及体外培养细胞系中的表达呈显著低水平，miR-150表达量低的患者放疗后缓解率低；转染miR-150 mimics能够增强辐射对NK-92细胞增殖的抑制作用和促进辐射诱导致凋亡作用，对NK/T细胞淋巴瘤有辐射增敏作用。

Objective: To investigate the expression of MicroRNA-150 (miR-150) in NK/T cell lymphoma tissues and its effect on radio-sensitivity of NK-92 cells. Methods: Thirty-six patients with NK/T cell lynphoma that treat-ed in Zhujiang Hospital Affiliated to Southern Medical University were included as study subjects, and their tissue samples were collected. All the patients received similar radiotherapy, and the short-term efficacy was evaluated by the standard of International Workgroup (IWG) of Lymphoma. The patients were further divided into CR (complete remission) group and Non-CR (non-complete remission) group according the treatment efficacy. The miR-150 ex-pression in lymphoma tissues and NK/T cell lymphoma cell lines were detected by qRT-PCR. The NK-92 cells were then transfected with miR-150 mimics. The effect of miR-150 mimics on NK-92 cell radiosensitivity was analyzed by MTT and colony formation assay; effect of miR-150 mimics on radiation induced apoptosis of NK-92 cells were analyzed by Flow Cytometry; and the effect of miR-150 mimics on the expression of apoptosis-related proteins (Cas-pase 3 and PARP) in NK-92 cells was determined by Western blotting. Results: According to IWG criteria, 12 pa-tients had CR while the other 24 patients had Non-CR. Compared with CR group, the microRNA-150 level in Non-CR group was significantly decreased(P<0.05). Compared with the normal sCD3 - CD56 + NK cells, miR-150 was sig-nificantly lower in NK/T cell lymphoma tissues (9.10 ±0.19 vs 4.01± 0.22 P<0.01 ) and five NK/T cell lymphoma cell lines (P<0.05); over-expression of miR-150 significantly decreased the proliferation and colony formation of NK-92 cells (all P<0.01), and increased the sensitivity enhancement ratio (SER) after radiation (the SER was 5.375 after 10 Gy exposure; in addition, over-expressions of miR-150 significantly promoted the radiation-induced apopto-sis of NK-92 cells [(37.3±1.24)% vs (28.3±2.34)%，P<0.05], and promoted the protein expression of caspase 3 and PARP in NK-92 cells. Conclusion: miR-150 expression significantly decreased in both NK/T cell lymphoms tissues and cell lines. The patients of low miR-150 expression had low CR after radiotherapy. miR-150 mimics transfection promoted radiation-induced apoptosis of NK-92 cells. miR-150 has an enhancement effect on radio-sensitivity of NK/T cell lynphoma.

国家自然科学基金青年项目资助(No.81400156)