目的：探讨表达NKG2D配体视黄酸早期转录因子1ε （retinoic acid early transcript 1ε，RAE1ε）的原B细胞株BaF3诱导产生的髓系抑制性细胞（myeloid-derived suppressor cell，MDSC）的杀伤功能及对NK细胞功能的影响。 方法： 以小鼠原B细胞株BaF3为基础，构建表达空质粒的BaF3-mock对照细胞和表达RAE1ε的BaF3-RAE1ε细胞。将BaF3-mock和BaF3-RAE1ε细胞分别注射小鼠后诱导产生CD11b + Gr-1 + MDSC，磁珠分选MDSC后与NK细胞共培养后，流式细胞术检测其对NK细胞表面NKG2D和CD107a表达的影响，ELISA法检测其对NK细胞分泌IFN-γ的影响。将MDSC分别与BaF3-mock和BaF3-RAE1ε细胞共培养后，乳酸脱氢酶释放法检测其对靶细胞BaF3-mock和BaF3-RAE1ε细胞的杀伤作用。 结果： 与BaF3-mock细胞相比，BaF3-RAE1ε细胞诱导产生的MDSC对NK细胞表面NKG2D和CD107a的表达没有明显影响（P＞0.05），对NK细胞分泌IFN-γ的水平也没有显著影响（P＞0.05）。与BaF3-mock细胞相比，BaF3-RAE1ε细胞诱导产生的MDSC对靶细胞BaF3-mock和BaF3-RAE1ε 的杀伤作用明显增强[ （1.99±0.39）% vs（8.63±1.45）%、 （5.09±0.67）% vs（17.33±0.41）%，P<0.01]。[ （1.20±0.09）% vs（10.31±0.69）%、 （5.52±1.64）% vs（18.91±3.04）%，P<0.01]。 结论： RAE-1ε增强MDSC对靶细胞的杀伤功能。

Objective: To study the cytolytic activity of myeloid-derived suppressor cell (MDSC) from BaF3-RAE1ε injected mice and its effect on the function of NK cell. Methods: Two derivatives of murine pro-B cell line BaF3 cells, expressing empty plasmid (termed BaF3-mock) or retinoic acid early transcript 1ε (RAE1ε, termed BaF3-RAE1ε) were respectively constructed in the previous study. CD11b + Gr-1 + MDSC magnetically purified from BaF3-mock or BaF3-RAE1ε bearing mice were co-cultured with NK cells. After 24 hours, the cells were harvested for detecting NKG2D and CD107a expression on NK cells by flow cytometry, and the supernatants were collected for detecting IFN-γ by ELISA. Moreover, MDSC sorted from BaF3-mock or BaF3-RAE1ε bearing mice were co-cultured with BaF3-mock or BaF3- RAE1ε target cells. After 5 h, the cytotoxicity of MDSC against BaF3-mock or BaF3-RAE1 target cells was evaluated by the lactate dehydrogenase release test. Results: There was no obvious dif-ference in secretion of IFN-γ by NK cells co-cultured with MDSC from BaF3-mock or BaF3-RAE1ε bearing mice (P＞0.05). No difference in NKG2D and CD107a expression was detected among NK cells co-cultured with MDSC from BaF3-mock or BaF3-RAE1ε bearing mice (P＞0.05). MDSC isolated from BaF3-RAE1ε bearing mice had higher direct cytolytic activity against BaF3-mock or BaF3-RAE1ε cells than MDSC isolated from BaF3-mock bear-ing mice (P<0.01). Conclusion: RAE1ε enhances the cytolytic activity of MDSC against BaF3-mock or BaF3-RAE1ε cells.

国家自然科学基金资助项目（No. 81373130，No.81001308）；江苏省自然科学基金资助项目（No. BK2010315）；扬州大学大学生创新创业训练计划项目。Project supported by the National Natural Science Foundation of China (No. 81373130，No.81001308)