目的: 研究黑色素瘤分化相关基因-7(melanoma differentiation associated gene 7, MDA-7)/IL-24对Burkitt淋巴瘤细胞的促分化作用并探讨其作用机制。 方法: 构建稳定过表达MDA-7/IL-24的人Burkitt淋巴瘤Raji和Daudi细胞株，MTS法检测稳定转染MDA-7/IL-24对Raji和Daudi细胞活力的影响；Transwell小室实验检测转染MDA-7/IL-24对Raji和Daudi细胞侵袭和迁移能力的影响；流式细胞术检测细胞凋亡水平及免疫表型；Western blotting技术检测转染MDA-7/IL-24对Raji和Daudi细胞表达分化相关蛋白Myb、BLIMP1及BCL-6的影响；建立裸鼠Raji细胞移植瘤模型，检测在体内环境中稳定转染MDA-7/IL-24对Raji细胞生物活性的影响。 结果: 过表达MDA-7/IL-24的Raji和Daudi细胞其增殖（P<0.05）、侵袭（P<0.01）及迁移（P<0.01）能力均明显下降，但凋亡细胞无明显增加（P>0.05），表达CD45及CD138的水平均明显增加（P<0.01），而表达CD10的水平明显下降（P<0.01）。过表达MDA-7/IL-24的Raji和Daudi细胞表达BLIMP1的水平明显增加（P<0.01），而表达Myb及BCL-6的水平均明显减低（P<0.01）。MDA-7/IL-24过表达组裸鼠模型Raji细胞移植瘤质量明显低于对照组[ （1.23±0.21）vs（1.96±0.24）g，P<0.01]。 结论: 转染MDA-7/IL-24可能通过诱导分化作用抑制Burkitt淋巴瘤细胞的生物活性。

Objective: To investigate the differentiation- inducing effect of melanoma differentiation associated gene 7（MDA-7）/IL-24 on human Burkitt lymphoma cells, as well as its underlying mechanism. Methods: Human Burkitt lymphoma cell lines (Raji and Daudi) that stably over-expressing MDA-7/IL-24 were constructed. The effect of MDA-7/IL-24 transfection on cell viability of Raji and Daudi cells was assayed by MTS method; the effect on mi-gration and invasion of Raji and Daudi cells were analyzed by Transwell assay. The apoptosis and immunopheno-types of Raji and Daudi cells with MDA-7/IL-24 over-expression were analyzed by flow cytometry. Meanwhile, the expressions of differentiation related proteins (Myb, BLIMP1 and BCL-6) were analyzed by Western blotting. Raji cell xenograft model was established on nude mice to analyze the effect of MDA-7/IL-24 on the bioactivity of Raji cells in vivo. Results: The cell viability, migration and invasion ability of Raji and Daudi cell lines that transfected with MDA-7/IL-24 were obviously decreased (all P<0.01), however, the apoptosis cells were not increased (P>0.05). The expressions of CD45 and CD138 were elevated (P<0.01) while the expression of CD10 was decreased (P<0.01) in Raji and Daudi cells overexpressing MDA-7/IL-24. MDA-7/IL-24 over-expression in Raji and Daudi cells significantly increased the expression of BLIMP1 (P<0.01), but decreased the expression of Myb and BCL-6(P＜0.01). The tumor mass of nude mice was significantly decreased after the treatment of MDA-7/IL-24 ([1.23±0.21] vs [1.96±0.24]g, P<0.01). Conclusion: Transfection with MDA-7/IL-24 inhibited the bio-actively of Burkitt lymphoma cells, which might be accomplished by inducing differentiation.

河北省卫计委指令性课题资助项目（No.20160171）