目的：探讨结肠癌患者组织中microRNA-99a表达水平以及对结肠癌细胞增殖和迁移的影响。 方法： 取南京医科大学附属常州二院胃肠病中心49例结肠癌患者肿瘤组织、癌旁组织（癌旁5cm）标本以及结肠癌细胞HCT-116、HT-29、SW-480、Ca-co-2和正常结肠上皮细胞HCoePic，采用实时荧光定量PCR 检测结肠癌患者肿瘤组织和结肠癌细胞中microRNA-99a表达水平；结肠癌细胞株HT-29转染microRNA-99a 抑制剂后，CCK-8法检测microRNA-99a对结肠癌细胞增殖的变化；transwell法观察mi-croRNA-99a对结肠癌细胞迁移的影响；Western blotting检测了HT-29中FGFR-3的表达水平。 结果： microRNA-99a表达在结肠癌组织中明显高于癌旁组织（6.27±0.48 vs 1.34±0.54, P<0.05）、在肿瘤细胞中明显高于正常结肠上皮细胞（5.48±0.34, 7.67±0.24,5.78±0.22, 6.28±0.44 vs 1.45±0.37, P<0.05）。转染microRNA-99a 抑制剂后，HT-29细胞的增殖和迁移能力均明显下降（P<0.05）；同时，HT-29中FGFR-3显著降低（P<0.05）。 结论： microRNA-99a在结肠癌组织中高表达，低表达microRNA-99a可减弱结肠癌细胞的增殖和迁移能力，且可能通过FGFR-3信号通路发挥作用。

Objective: To investigate the expression of microRNA-99a in the tumor tissues of colon cancer patients and its effect on cancer cell proliferation and migration. Methods: 49 pairs of cancer tissue and adjacent tissue (5 cm away from cancer tissue) from colon cancer patients that treated in Gastrointestinal Center of Affiliated Chang-zhou Second Hospital of Nanjing Medical University, and colon cancer cell lines (HT-29，HCT-116，SW480，Caco-2) as well as normal epithelial HcoePic cell line were selected for our research. Quantitative real-time PCR was used to detect the levels of microRNA-99a in tumor tissues, adjacent tissues and tumor cells; After transfection of mc-iroRNA-99a inhibitor, we used CCK-8 to test the cell proliferation, transwell assay to observe the cell migration,and Western lotting to examine the levels of FGFR3 in HT-29 cells. Results: The expression of microRNA-99a in the cancer tissues was significantly higher than that in normal para-cancerous tissues (6.27±0.48 vs 1.34±0.54, P<0.05), and its expression in tumor cells was significantly higher than that in normal colon epithelial cells（5.48±0.34, 7.67±0.24, 5.78±0.22, 6.28±0.44 vs 1.45±0.37, P<0.05). After microRNA-99a inhibitor transfection, cell pro-liferation and migration of HT-29 cells were significantly decreased (P<0.05); , in the meanwhile, the mRNA and protein levels of FGFR3 were significantly decreased in HT-29 cells (P<0.05). Conclusion: microRNA-99a was highly expressed in the tumor tissue of colon cancer patients and colon cancer cells, and low expression of microR-NA-99a may weaken the proliferation and migration ability of cancer cells, which might be accomplished throughFGFR3 signaling pathway.

常州市科技基础研究计划资助（No. CJ20122014）