目的: 探讨lncRNA NCRNA00173在骨肉瘤组织中表达及其临床意义。 方法: 通过实时荧光定量PCR法检测l-ncRNANCRNA00173在54例骨肉瘤组织及癌旁组织标本（收集自四川省人民医院骨科2012年10月至2016年12月手术患者）中的表达，分析其表达的临床意义。 结果: 与癌旁组织比较，lncRNA NCRNA00173在骨肉瘤组织标本中的表达明显降低（1.793±0.158 vs 5.368±0.285，t=10.96，P<0.01）。lncRNA NCRNA00173的表达与疾病分期、淋巴结转移、远处转移、肿瘤分化及生存状态明显相关(均P<0.05)，而与患者的性别、年龄无关 (均P>0.05)；此外,高表达lncRNANCRNA00173患者的OS及PFS均较低表达者明显延长（P<0.01）。Cox多因素回归模型分析提示，lncRNA NCRNA00173的表达、远处转移及临床分期是骨肉瘤独立的预后因素(P<0.05)。 结论 : ncRNANCRNA00173参与调节骨肉瘤的发生发展，可作为潜在的骨肉瘤诊断和预后评估的分子标志物。

Objective: To investigate the expression and clinical significance of lncRNA NCRNA00173 in the tis-sues of osteosarcoma patients. Methods: The expression of lncRNA NCRNA00173 mRNA was detected in 54 cases of osteosarcoma tissues and adjacent tissues (collected from the patients underwent surgery between October, 2012 and December, 2016 at the Department of Orthopedic, Sichuan Provincial People’s Hospital) by real-time fluores-cence quantification PCR, and its clinical significance was analyzed. Results: The expression of lncRNA NCRNA00173 in osteosarcoma tissues was obviously decreased compared with that in paracarcinoma tissues (1.793±0.158 vs 5.368±0.285，t=10.96，P<0.01). LncRNA NCRNA00173 expression was not significantly correlat-ed with patients’gender or age (both P>0.05), but remarkably correlated with disease stage, distant metastasis, tu-mor differentiation and the survival (all P<0.05); Furthermore, patients with high lncRNA NCRNA00173 expres-sion had significant longer OS and median PFS. Cox multivariate regression analysis suggested that lncRNA NCRNA00173 expression, distant metastasis and clinical stage were the independent prognostic factors for osteosar-coma (P<0.05). Conclusion: lncRNA NCRNA00173 is involved in the occurrence and development of osteosarco-ma, and can be used as a molecular marker for the diagnosis and prognosis of osteosarcoma.

四川省科技支撑项目资助（No.2012SZ0181）