目的: 探讨结直肠差异表达长链非编码RNA CRNDE（colorectal neoplasia differentially expressed）在非小细胞肺癌（NSCLC）组织标本中的表达及其临床意义。 方法: 收集2011年1月至2015年12月成都军区总医院行根治性或姑息性切除术的NSCLC患者137例，以相应癌旁组织和29例肺外伤非肺癌患者的正常肺组织作为对照，通过实时荧光定量PCR法检测lncRNA CRNDE在癌组织及癌旁组织标本中的表达，分析其表达与患者临床病理特征及预后的关系。 结果: lncRNA CRNDE在肺癌癌组织中的平均表达水平为5.283±0.245，与非肿瘤组织（1.098±0.082）比较，差异有统计学意义(t=14.59，P<0.01)。lncRNA CRNDE在肺癌组织标本中的表达较癌旁组织明显增高。 lncRNA CRNDE的表达与患者的年龄、性别、吸烟史、肿瘤大小、淋巴结转移及肿瘤分化无关(均P>0.05)；与疾病分期、远处转移、病理类型及生存状态明显相关(均P<0.05)。lncRNA CRNDE高表达水平与NSCLC 中的病理类型相关（P<0.05），在腺癌组的表达水平明显高于鳞癌及其他类型组的表达水平；此外, 高表达 lncRNA CRNDE的患者的PFS为(20.00±1.72)个月，较低表达者(34.07±1.97)缩短，差异有统计学意义（χ 2 =15.940，P<0.01）；低表达lncRNACRNDE的患者的OS为(47.50±2.31)个月,较高表达者(32.15±2.19)明显延长，差异有统计学意义（χ 2 =12.40，P<0.01）。Cox多因素回归模型分析显示, lncRNA CRNDE的表达、远处转移及临床分期是NSCLC独立的预后因素(P<0.05)。 结论: lncRNA CRNDE参与调节NSCLC的发生发展，可作为潜在的NSCLC诊断和预后评估的生物标志物。

Objective: To investigate the expression and clinical significance of lncRNA CRNDE (colorectal neo-plasia differentially expressed) in NSCLC(non-small cell lung cancer) tissues. Methods: 137 cases of NSCLC tis-sues and corresponding para-cancerous tissues from patients with NSCLC who underwent radical or palliative resec-tion in General Hospital of Chengdu Military Region from January 1，2011 to December 31, 2015 were collected for this study; Meanwhile, 29 cases of normal lung tissues from patients suffered from traumatic injury were used as controls. The expression of lncRNA CRNDE in 137 cases of NSCLC tissues and adjacent tissues were detected by QRT-PCR method, and the relationship between its expression and clinical pathological characteristics and progno-sis of NSCLC were analyzed. Results: Compared with the para-cancerous tissues (1.098±0.082), the average expres-sion level of lncRNA CRNDE in cancer tissues (5.283±0.245) was significantly higher with statistical significance (t=14.59, P<0.001). lncRNA CRNDE expression was significantly correlated with disease stage, distant metastasis,pathological type and survival status (all P<0.05), but not associated with age, gender, smoking, tumor size, lymph node metastasis and tumor difference (all P>0.05). The high lncRNA CRNDE expression was related to the patho-logical type of NSCLC (P< 0.05), and the expression of lncRNA CRNDE in adenocarcinoma group was significant-ly higher than that in squamous cell carcinoma and other groups. Moreover, Kaplan-Meier analysis demonstrated that the progression-free survival time in patients with high lncRNA CRNDE expression (20±1.72 months) was shortened compared with the patients with low expression (34.07±1.97 months), the difference was statistically sig-nificant (χ 2 =15.940, P<0.001); the overall survival time of patients with low lncRNA CRNDE expression (47.50±2.31 months) was significantly prolonged compared with those patients with high expression (32.15±2.19 months),the difference was statistically significant (χ 2 =12.40, P=0.004). Cox multivariate survival analysis also indicated that lncRNA CRNDE expression, distant metastasis, clinical stage were the independent prognostic markers for NSCLC (P<0.05). Conclusion: lncRNA CRNDE is involved in the occurrence and development of NSCLC, and can be used as a biomarker for the diagnosis and prognosis of non-small cell lung cancer.

国家自然科学基金资助项目（NO.81171869）